使用电子显微镜研究肾小球超微结构的样本制备和体视学方法

Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy.

作者信息

White Kathryn E

机构信息

EM Research Services, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK.

出版信息

Methods Mol Biol. 2020;2067:89-102. doi: 10.1007/978-1-4939-9841-8_7.

DOI:10.1007/978-1-4939-9841-8_7

PMID:31701447

Abstract

In this chapter we describe conventional methods used for preparing renal tissue for transmission electron microscopy. We also describe a relatively new technique, serial block face scanning electron microscopy. Protocols are given for processing, sectioning, and imaging of tissue along with methods for obtaining quantitative data from the results.

摘要

在本章中，我们描述了用于透射电子显微镜检查的肾组织制备常规方法。我们还描述了一种相对较新的技术，即连续块面扫描电子显微镜。文中给出了组织处理、切片和成像的方案，以及从结果中获取定量数据的方法。