Wójcik-Borowska Katarzyna, Wójciak Weronika, Żuk Magdalena, Luchowski Piotr, Skalska-Kamińska Agnieszka, Pacuła Wiktoria, Sowa Ireneusz, Wójciak Magdalena
Department of Child Neurology, Medical University of Lublin, 20-093 Lublin, Poland.
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Molecules. 2025 Jun 9;30(12):2515. doi: 10.3390/molecules30122515.
Polyphenols are valuable contributors to skin health, offering potent antioxidant and anti-inflammatory effects that help counteract the process of inflammaging. According to the literature, L. is a rich source of polyphenolic compounds, suggesting its potential for applications in cosmetology and dermatology. This study aimed to evaluate the antioxidant and anti-inflammatory activity of polyphenol-rich fractions isolated from leaves (UdLs) and flowers (UdFs) using human skin cells subjected to oxidative stress and lipopolysaccharide (LPS) stimulation, respectively. Extracts were obtained via an accelerated solvent extraction and further purified by a solid-phase extraction to concentrate their polyphenolic content. Their chemical composition was analyzed using UPLC-DAD-MS. Biological activity was assessed through cytotoxicity assays (NR and MTT), chemical and cellular antioxidant assays (DPPH, ABTS, FRAP, CUPRAC, TPC, and H₂DCFDA), an evaluation of antioxidant enzyme activity (SOD, CAT), lipid peroxidation (MDA), and cytokine production (IL-1β, IL-6, IL-10). Our study showed that both fractions were abundant in phenolic compounds, with chlorogenic acid identified as the predominant constituent. UdLs contained higher levels of phenolic acids, whereas the UdF was richer in flavonoids, particularly derivatives of isorhamnetin. Both the UdL and UdF were non-cytotoxic and exhibited strong radical scavenging potential, with the UdL being slightly more effective. They significantly reduced intracellular ROS levels, enhanced the activity of antioxidant enzymes, and attenuated lipid peroxidation in cells exposed to oxidative stress. Moreover, both fractions reduced the secretion of pro-inflammatory cytokines in LPS and HO-stimulated fibroblasts. These results highlight the potential of polyphenolic fractions derived from leaves and flowers as multifunctional ingredients for anti-aging and skin-protective cosmetics.
多酚对皮肤健康有益，具有强大的抗氧化和抗炎作用，有助于对抗炎症衰老过程。根据文献记载，[植物名称]是多酚类化合物的丰富来源，表明其在美容和皮肤科领域的应用潜力。本研究旨在分别使用遭受氧化应激的人类皮肤细胞和脂多糖（LPS）刺激的人类皮肤细胞，评估从[植物名称]叶子（UdLs）和花朵（UdFs）中分离出的富含多酚的组分的抗氧化和抗炎活性。通过加速溶剂萃取获得提取物，并通过固相萃取进一步纯化以浓缩其多酚含量。使用超高效液相色谱-二极管阵列检测器-质谱联用仪（UPLC-DAD-MS）分析其化学成分。通过细胞毒性试验（NR和MTT）、化学和细胞抗氧化试验（DPPH、ABTS、FRAP、CUPRAC、TPC和H₂DCFDA）、抗氧化酶活性评估（SOD、CAT）、脂质过氧化（MDA）和细胞因子产生（IL-1β、IL-6、IL-10）来评估生物活性。我们的研究表明，两种组分都富含酚类化合物，其中绿原酸被确定为主要成分。UdLs中酚酸含量较高，而UdF中黄酮类化合物含量更丰富，尤其是异鼠李素衍生物。UdL和UdF均无细胞毒性，并表现出强大的自由基清除潜力，其中UdL的效果稍好。它们显著降低了细胞内活性氧水平，增强了抗氧化酶的活性，并减轻了遭受氧化应激的细胞中的脂质过氧化。此外，两种组分都减少了LPS和HO刺激的成纤维细胞中促炎细胞因子的分泌。这些结果突出了从[植物名称]叶子和花朵中提取的多酚组分作为抗衰老和皮肤保护化妆品多功能成分的潜力。
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