Buacheen Pensiri, Karinchai Jirarat, Inthachat Woorawee, Butkinaree Chutikarn, Wongnoppawich Ariyaphong, Imsumran Arisa, Temviriyanukul Piya, Inoue Yoshihiro H, Pitchakarn Pornsiri
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Muang Chiang Mai, Chiang Mai 50200, Thailand.
Institute of Nutrition, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
Int J Mol Sci. 2025 Jun 13;26(12):5694. doi: 10.3390/ijms26125694.
Aging is a significant risk factor for various conditions, including neurodegeneration, cardiovascular disease, and type 2 diabetes. The accumulation of reactive oxygen species (ROS) and a decline in antioxidant defense are mechanisms that are widely acknowledged as causing the acceleration of both aging and the onset of age-related diseases. To promote longevity and reduce the risk of the development of aging-related disorders, it is essential to prevent or minimize oxidative stress and enhance antioxidant defense. It has been shown that (AB), a jewel orchid rich in phenolic compounds, can impact various biological activities associated with aging prevention. These activities include antioxidant, anti-inflammation, anti-insulin resistance, and anti-obesity effects. The aim of this study was to explore whether AB extract (ABE) could serve as an anti-aging agent using a -deficient model, which accelerates the process of aging through ROS production. The results demonstrated that ABE, at a concentration of 2.5 mg/mL, significantly extended the lifespan of the flies and helped maintain their locomotor activity as they aged. ABE also reduced the age-related accumulation of damaged proteins in the muscle of the flies by inhibiting the expression of , a genetic marker for oxidative stress. This finding agrees with those from in vitro experiments, which have shown the potential for ABE to reduce the production of ROS induced by HO in myoblasts. ABE has been shown to attenuate insulin resistance, an age-related disorder, by inhibiting the pro-inflammatory cytokine TNF-α, which in turn increased insulin-stimulated glucose uptake in adipocytes. These findings suggest a promising role of ABE as an ingredient in functional foods or nutraceuticals aimed at promoting health, preventing oxidative stress, and potentially managing age-associated diseases.
衰老 是多种疾病的重要风险因素，包括神经退行性疾病、心血管疾病和2型糖尿病。活性氧（ROS）的积累和抗氧化防御能力的下降是被广泛认为导致衰老加速和与年龄相关疾病发生的机制。为了促进长寿并降低与衰老相关疾病的发生风险，预防或最小化氧化应激并增强抗氧化防御至关重要。已表明，一种富含酚类化合物的宝石兰（AB）可影响与预防衰老相关的各种生物活性。这些活性包括抗氧化、抗炎、抗胰岛素抵抗和抗肥胖作用。本研究的目的是使用一种通过产生ROS加速衰老过程的缺陷模型，探索AB提取物（ABE）是否可作为一种抗衰老剂。结果表明，浓度为2.5 mg/mL的ABE显著延长了果蝇的寿命，并在果蝇衰老过程中帮助维持其运动活性。ABE还通过抑制作为氧化应激遗传标记物的的表达，减少了果蝇肌肉中与年龄相关的受损蛋白质积累。这一发现与体外实验结果一致，体外实验表明ABE有潜力减少成肌细胞中由HO诱导的ROS产生。已表明ABE通过抑制促炎细胞因子TNF-α来减轻胰岛素抵抗（一种与年龄相关的疾病），这反过来又增加了脂肪细胞中胰岛素刺激的葡萄糖摄取。这些发现表明ABE作为功能性食品或营养保健品中的一种成分，在促进健康、预防氧化应激以及潜在地管理与年龄相关疾病方面具有广阔前景。
