Duan Wen, Zheng Bisheng, Li Tong, Liu Rui Hai
School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China.
Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health, School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China.
Nutrients. 2025 Jun 9;17(12):1962. doi: 10.3390/nu17121962.
BACKGROUND/OBJECTIVES: Oats and oat bran are rich in dietary fiber, polyphenols and other phytochemicals.
In this study, we evaluated the phytochemical content and established LPS-induced RAW 264.7 macrophage inflammation and DSS-induced Caco-2 cell inflammation models to investigate the anti-inflammatory activities of oat and oat bran polyphenols and their molecular mechanisms.
The results showed that oat and oat bran polyphenols (free and bound polyphenols) enhanced phagocytosis, decreased the expression of nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), reduced the production of NO and ROS, increased the mitochondrial membrane potential, and reduced the inflammatory cytokines (TNF-α, IL-1β, and IL-6) at the gene level in the RAW 264.7 macrophage inflammation model induced by LPS expression, thus demonstrating strong anti-inflammatory activity. In Caco-2 cells, oat and oat bran polyphenols pretreatment attenuated the DSS-induced decrease in trans-epithelial electron resistance value, increased tight junction protein expression, and reduced cell permeability in Caco-2 cell monolayers, which in turn reduced inflammatory damage in the organism.
In summary, the present study not only reveals the mechanism by which oat and oat bran polyphenols inhibit macrophage inflammation and impairment of intestinal barrier function at defined concentration in vitro, but also highlights potential for oat bran as a functional food.
背景/目的：燕麦和燕麦麸富含膳食纤维、多酚及其他植物化学物质。
在本研究中，我们评估了植物化学成分，并建立了脂多糖诱导的RAW 264.7巨噬细胞炎症模型和葡聚糖硫酸钠诱导的Caco - 2细胞炎症模型，以研究燕麦和燕麦麸多酚的抗炎活性及其分子机制。
结果表明，燕麦和燕麦麸多酚（游离和结合多酚）在脂多糖诱导的RAW 264.7巨噬细胞炎症模型中，增强了吞噬作用，降低了一氧化氮合酶（iNOS）和环氧化酶 - 2（COX - 2）的表达，减少了NO和ROS的产生，增加了线粒体膜电位，并在基因水平上降低了炎症细胞因子（TNF -α、IL - 1β和IL - 6），从而显示出强大的抗炎活性。在Caco - 2细胞中，燕麦和燕麦麸多酚预处理减弱了葡聚糖硫酸钠诱导的跨上皮电阻值降低，增加了紧密连接蛋白表达，并降低了Caco - 2细胞单层的细胞通透性，进而减少了机体的炎症损伤。
综上所述，本研究不仅揭示了燕麦和燕麦麸多酚在体外特定浓度下抑制巨噬细胞炎症和肠道屏障功能损伤的机制，还突出了燕麦麸作为功能性食品的潜力。
