National Engineering Research Center for Functional Food, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Lipid Technology and Engineering, School of Food Science and Engineering, Henan University of Technology, Zhengzhou 450001, China.
Food Res Int. 2022 Jun;156:111142. doi: 10.1016/j.foodres.2022.111142. Epub 2022 Mar 15.
The carbonyl trapping activity of bound-polyphenol rich insoluble dietary fiber (BP-IDF) from different whole grains and underlying mechanism of these BP-IDF actions were studied under simulated physiological conditions. We found that the black highland barley BP-IDF exhibited the most pronounced effect in scavenging carbonyls by trapping 88.7%, 72.2%, 95.7%, and 31.4% for methylglyoxal, glyoxal, acrolein, and malondialdehyde within 24 h, respectively. After vitro gastrointestinal digestion, the black highland barley BP-IDF still retained considerable trapping activity for carbonyls. The carbonyl scavenging capacity was reduced by up to 93% after removing bound polyphenols from the black highland barley BP-IDF, which was consistent with the reduction in its total phenolic content. Moreover, the formation of adducts between reactive carbonyl species (RCS) and polyphenols bound to insoluble dietary fiber (IDF) was also detected. Overall, these findings confirmed that IDF-bound polyphenols were still active to trap RCS, indicating the potential benefits of BP-IDF from whole grains as functional ingredients to limit carbonyl stress across the gastrointestinal tract.
在模拟生理条件下，研究了来自不同全谷物的结合多酚丰富的不溶性膳食纤维（BP-IDF）的羰基捕获活性及其作用机制。我们发现，黑青稞 BP-IDF 在 24 小时内对甲基乙二醛、乙二醛、丙烯醛和丙二醛的清除效果最为显著，分别捕获了 88.7%、72.2%、95.7%和 31.4%的羰基。经过体外胃肠消化后，黑青稞 BP-IDF 仍保留相当大的捕获羰基的能力。从黑青稞 BP-IDF 中去除结合多酚后，其对羰基的清除能力最高降低了 93%，这与总酚含量的降低一致。此外，还检测到反应性羰基物质（RCS）与不溶性膳食纤维（IDF）结合的多酚之间加合物的形成。总的来说，这些发现证实了 IDF 结合的多酚仍然具有捕获 RCS 的活性，表明全谷物来源的 BP-IDF 作为功能性成分在整个胃肠道中限制羰基应激的潜在益处。
