血浆游离氰化物和血液总氰化物：一种快速全自动微蒸馏测定法。

Plasma free cyanide and blood total cyanide: a rapid completely automated microdistillation assay.

作者信息

Groff W A, Stemler F W, Kaminskis A, Froehlich H L, Johnson R P

出版信息

J Toxicol Clin Toxicol. 1985;23(2-3):133-63. doi: 10.3109/15563658508990623.

Abstract

Techniques are presented which provide direct measurement of both free cyanide (CN-) in plasma and total CN- in whole blood. Loss of total CN- from blood is prevented by conversion to cyanmethemoglobin. Both free and total CN- are assayed by a completely automated method providing readout 17 minutes after sampling. No prior isolation technique is required and sensitivity is adjustable to cover a broad range of CN- concentrations from 1 to 4000 uM. Precision of blood CN- values from 2 to 2500 uM is within +/- 2.3%. No interference results from thiocyanate or thiosulfate at a concentration of approximately 1 mM.

摘要

本文介绍了一些技术，这些技术可直接测量血浆中的游离氰化物（CN-）和全血中的总CN-。通过将其转化为氰化高铁血红蛋白可防止血液中总CN-的损失。游离CN-和总CN-均通过完全自动化的方法进行测定，采样后17分钟即可读出结果。无需事先进行分离技术，灵敏度可调节，以覆盖1至4000 μM的广泛CN-浓度范围。2至2500 μM血液CN-值的精密度在+/- 2.3%以内。浓度约为1 mM的硫氰酸盐或硫代硫酸盐不会产生干扰。