López Sofía, Álvarez Ignacio, Andreoli V, Delgado S, Perez S, Pereyra S, Romeo F, Grolli S, Verna Andrea Elizabeth
Instituto Nacional de Tecnología Agropecuaria, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible, Ruta 226, km 73.5, Balcarce B7620, Buenos Aires, Argentina.
Division of Ruminant Medicine, Department of Clinical Sciences, Swedish University of Agricultural Sciences, P.O. Box 7054, 756 51 Uppsala, Sweden.
Viruses. 2025 May 23;17(6):744. doi: 10.3390/v17060744.
Uterine diseases in cattle are frequently linked to bacterial infections, with pathogens commonly isolated from the uterine lumen. Bovine Gammaherpesvirus Type 4 (BoGHV-4) is notably prevalent in certain regions of Argentina and is associated with uterine diseases in postpartum cattle. This study aims to evaluate the impact of platelet-rich plasma (PRP) on the gene expression related to BoGHV-4 infection in the presence of lipopolysaccharide (LPS), exploring the potential of PRP as a therapeutic alternative. The interaction between LPS and Toll-like receptor 4 (TLR4) plays a crucial role in inflammatory responses, triggering cytokine production and immune activation. Our results show that PRP modulates TLR4 and TNF-α gene expression, indicating a potential inhibitory role in inflammatory processes. Furthermore, PRP alter the temporal dynamics of BoGHV-4 replication by modulating the expression of the viral immediate-early gene (IE-2) and delaying proinflammatory cytokine responses such as IL-8. Notably, PRP enhances IFN-γ expression, which could help prevent tissue damage caused by bacterial and viral coinfection. These findings highlight the potential of PRP as an anti-inflammatory agent with therapeutic benefits in treating uterine diseases, offering an alternative to traditional antibiotic treatments.
牛的子宫疾病通常与细菌感染有关，病原体常从子宫腔中分离出来。牛4型伽马疱疹病毒（BoGHV-4）在阿根廷的某些地区尤为普遍，并且与产后母牛的子宫疾病有关。本研究旨在评估富含血小板血浆（PRP）在脂多糖（LPS）存在的情况下对与BoGHV-4感染相关的基因表达的影响，探索PRP作为一种治疗选择的潜力。LPS与Toll样受体4（TLR4）之间的相互作用在炎症反应中起着关键作用，触发细胞因子产生和免疫激活。我们的结果表明，PRP调节TLR4和TNF-α基因表达，表明其在炎症过程中可能具有抑制作用。此外，PRP通过调节病毒立即早期基因（IE-2）的表达并延迟促炎细胞因子反应（如IL-8）来改变BoGHV-4复制的时间动态。值得注意的是，PRP增强IFN-γ表达，这有助于预防由细菌和病毒合并感染引起的组织损伤。这些发现突出了PRP作为一种抗炎剂在治疗子宫疾病方面具有治疗益处的潜力，为传统抗生素治疗提供了一种替代方法。
