Peng Ning, Guo Songtao, Tang Yanmei, Li Shicheng, Pham Trang, Kuang Xianyan, Yi Zili, Xiao Liang
College of Bioscience & Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Department of Natural Resources and Environmental Sciences, Alabama A&M University, Huntsville, AL 35801, USA.
Plants (Basel). 2025 Jun 9;14(12):1760. doi: 10.3390/plants14121760.
We aim to provide a theoretical basis for improving the cold tolerance of spp., a widely recognized C4 perennial bioenergy crop, and extending its application in the industry. This study evaluated its cold tolerance by measuring the relative electrical conductivity (REC) of detached leaves. We calculated the half-lethal temperature (LT) during non-acclimation and acclimation treatments in the 12 wild genotypes of and from different regions of China. In this study, five temperature treatments were carried out to simulate the natural early spring cold process, with temperatures of 0 °C, -4 °C, -8 °C, -12 °C, and-16 °C. We compared the REC and LT during the non-acclimation and acclimation treatments, and the results show that the REC of the 12 genotypes increased with a decrease in the treatment temperature, forming an S-shaped curve, which was significantly negatively correlated with the corresponding temperature. Under non-cold acclimation, the B0111 from Jiamusi, Heilongjiang Province, had the lowest LT of -9.49 °C, showing extraordinarily strong cold tolerance. However, A0630 from Shaoxing, Zhejiang Province, had the highest LT of -6.43 °C, demonstrating the weakest cold tolerance. After 21 days of cold acclimation, B0111 still exhibited the most substantial cold tolerance. While A0630 showed an enhanced cold tolerance, it remains the weakest in this study. The cold acclimation abilities of the 12 genotypes varied from -0.016 to 0.666 °C. Additionally, we found that the tolerance abilities of were enhanced after acclimation. Furthermore, its tolerance capacity was found to vary according to the geographic factor of its original location, which shows the significance of the correlation with latitude. However, there was no correlation found with altitude or longitude.
我们旨在为提高一种广泛认可的C4多年生生物能源作物——[作物名称未给出]的耐寒性并扩大其在工业中的应用提供理论依据。本研究通过测量离体叶片的相对电导率（REC）来评估其耐寒性。我们计算了来自中国不同地区的12个野生[作物名称未给出]基因型在非驯化和驯化处理期间的半致死温度（LT）。在本研究中，进行了五种温度处理以模拟自然早春的寒冷过程，温度分别为0℃、-4℃、-8℃、-12℃和-16℃。我们比较了非驯化和驯化处理期间的REC和LT，结果表明12个基因型的REC随着处理温度的降低而增加，形成一条S形曲线，与相应温度呈显著负相关。在非冷驯化条件下，来自黑龙江佳木斯的[作物名称未给出]B0111的LT最低，为-9.49℃，表现出极强的耐寒性。然而，来自浙江绍兴的[作物名称未给出]A0630的LT最高，为-6.43℃，表明其耐寒性最弱。经过21天的冷驯化后，B0111仍然表现出最强的耐寒性。虽然A0630的耐寒性有所增强，但在本研究中仍然是最弱的。12个基因型的冷驯化能力在-0.016至0.666℃之间变化。此外，我们发现[作物名称未给出]驯化后的耐受能力有所增强。此外，其耐受能力因原生长地的地理因素而异，这表明与纬度的相关性具有重要意义。然而，未发现与海拔或经度存在相关性。
