Wang Zhi-Lei, Wu Dong, Hui Miao, Wang Ying, Han Xing, Yao Fei, Cao Xiao, Li Yi-Han, Li Hua, Wang Hua
College of Enology, Northwest A&F University, Yangling, Shaanxi, China.
College of Life Science, Langfang Normal University, Langfang, Hebei, China.
Front Plant Sci. 2022 Nov 24;13:1014330. doi: 10.3389/fpls.2022.1014330. eCollection 2022.
The goals of this work were to screen physiological and biochemical indexes to assess a set of germplasm resources, to compare evaluation methods for cold hardiness, and to establish a comprehensive method that can be used for more accurate screening for cold hardiness in Four single methods were used to evaluate the cold hardiness of 20 germplasms resources and 18 physiological and biochemical indexes related to cold hardiness were determined. The LT values determined by electrical conductivity (EL), 2,3,5-triphenyltetrazolium chloride staining (TTC), differential thermal analysis (DTA), and recovery growth (RG) methods showed extremely significant positive correlation. Bound water content (BW), proline content (Pro), total soluble sugar content (TSS), malondialdehyde content (MDA), catalase content (CAT), and ascorbic acid content (ASA) exhibited significant correlation with LT values measured by different evaluation methods. The comprehensive cold hardiness index calculated by principal component analysis (PCA) combined with subordinate function (SF) was negatively correlated with LT values measured by different evaluation methods. Meili and Ecolly exhibited the highest cold hardiness, indicating their potential for use as parents for cold hardiness breeding. EL, DTA, TTC, and RG methods successfully distinguished cold hardiness among different germplasm lines. Measurements of BW, Pro, TSS, MDA, CAT, and ASA in dormant shoots also can be used as main physiological and biochemical indexes related to cold hardiness of . Comprehensive evaluation by PCA combined with SF can accurately screen cold hardiness in . This study provides a reference and accurate identification method for the selection of cold hardiness parents and the evaluation of cold hardiness of germplasm of .
这项工作的目标是筛选生理生化指标以评估一组种质资源，比较抗寒性的评估方法，并建立一种可用于更准确筛选抗寒性的综合方法。使用四种单一方法评估20种种质资源的抗寒性，并测定了18个与抗寒性相关的生理生化指标。通过电导率（EL）、2,3,5-氯化三苯基四氮唑染色（TTC）、差热分析（DTA）和恢复生长（RG）方法测定的LT值显示出极显著的正相关。束缚水含量（BW）、脯氨酸含量（Pro）、总可溶性糖含量（TSS）、丙二醛含量（MDA）、过氧化氢酶含量（CAT）和抗坏血酸含量（ASA）与通过不同评估方法测得的LT值呈现显著相关性。通过主成分分析（PCA）结合隶属函数（SF）计算的综合抗寒指数与通过不同评估方法测得的LT值呈负相关。美丽和埃科利表现出最高的抗寒性，表明它们作为抗寒育种亲本的应用潜力。EL、DTA、TTC和RG方法成功区分了不同种质系之间的抗寒性。休眠芽中BW、Pro、TSS、MDA、CAT和ASA的测定也可作为与抗寒性相关的主要生理生化指标。PCA结合SF的综合评估可以准确筛选抗寒性。本研究为抗寒亲本的选择和种质抗寒性的评估提供了参考和准确的鉴定方法。
