Watanabe Riku, Artigas Ramirez Maria Daniela, Agake Shin-Ichiro, Bellingrath-Kimura Sonoko Dorothea, Lewandowska Sylwia, Onishi Yuki, Nishikawa Yohei, Takeyama Haruko, Yasuda Michiko, Ohkama-Ohtsu Naoko
Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8, Saiwai-cho, Fuchu 183-8509, Tokyo, Japan.
College of Biological Science and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Haidian District, Beijing 100083, China.
Plants (Basel). 2025 Jun 11;14(12):1786. doi: 10.3390/plants14121786.
Soybeans have been cultivated in Poland for more than 140 years. However, Poland's cold and water-deficient climatic conditions hinder soybean cultivation. Although the availability of suitable soybean varieties in Poland contributes to meeting the demand for soybean production, it is important to identify rhizobial inoculants in Polish soils suitable for soybean cultivation. In this study, we cultivated soybean varieties (Abelina, Merlin, and Sultana) grown in soils taken from four regions in Poland and isolated 330 strains from soybean root nodules. 16S rRNA gene sequencing identified 49 strains of highly stress-tolerant nodule-associated bacteria, including , , , , and spp. Several isolates exhibited positive effects on soybean growth under cold and water-deficient conditions. In particular, the isolate PSN49, which is phylogenetically similar to . USDA 123, increased plant biomass and nodule formation in the soybean cultivar Abelina under abiotic stress conditions due to its high nitrogen-fixing activity. Whole-genome comparisons between PSN49 and other strains revealed that trehalose biosynthesis genes and cold shock proteins contributed to cold stress tolerance. These findings and the strains identified in this study will enhance soybean production and deepen the understanding of the soybean-rhizobium relationship in Poland.
大豆在波兰的种植历史已超过140年。然而，波兰寒冷且缺水的气候条件阻碍了大豆种植。尽管波兰有适合的大豆品种有助于满足大豆生产需求，但确定波兰土壤中适合大豆种植的根瘤菌接种剂很重要。在本研究中，我们种植了在波兰四个地区采集的土壤中生长的大豆品种（阿贝丽娜、梅林和苏丹娜），并从大豆根瘤中分离出330株菌株。16S rRNA基因测序鉴定出49株高度耐胁迫的根瘤相关细菌，包括[具体细菌种类未给出]等。一些分离株在寒冷和缺水条件下对大豆生长表现出积极影响。特别是，与[具体细菌名称未给出]USDA 123系统发育相似的分离株PSN49，由于其高固氮活性，在非生物胁迫条件下增加了大豆品种阿贝丽娜的植株生物量和根瘤形成。PSN49与其他[具体细菌名称未给出]菌株的全基因组比较表明，海藻糖生物合成基因和冷休克蛋白有助于耐冷胁迫。这些发现以及本研究中鉴定出的菌株将提高大豆产量，并加深对波兰大豆 - 根瘤菌关系的理解。
