Heidar Beygi Somayeh, Namazi Gholamreza, Asa Parastoo, Salami Raziyeh, Mafi Alireza, Raygan Fariba
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran.
Department of Clinical Biochemistry, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Caspian J Intern Med. 2025 Mar 11;16(2):255-262. doi: 10.22088/cjim.16.2.255. eCollection 2025.
Oxidative stress has been well established to participate in the pathology of coronary artery disease (CAD). Sestrin family of proteins have recently emerged as important suppressors of oxidative stress. However, only few studies have reported the levels of sestrins and their clinical significance in CAD patients.
Participants were ninety patients referred to the cardiac angiography unit for cardiac angiography. Thirty-two subjects were diagnosed as having stable CAD, twenty patients had unstable CAD and thirty-eight subjects had no CAD. All patients underwent angiography and the severity of coronary stenosis was calculated by modified Gensini score. The levels of glucose, triglyceride, total cholesterol, HDL, LDL, hs-CRP and hematological parameters were determined in the fasting blood samples by routine methods. Plasma levels of sestrin-1 and sestrin-2 were measured by ELISA.
Although the plasma levels of sestrin-1 were significantly lower in both case groups compared with the control group (P<0.001), there were no significant differences in sestrin-1 levels between the two patient groups. The levels of sestrin-2 were also significantly lower in both CAD groups than in controls (P=0.001), but no significant difference was found between stable and unstable patients. In the whole study subjects, plasma sestrin-1 and sestrin-2 showed negative correlation with the coronary artery score. By multivariate analysis only sestrin-2 levels were significantly related to CAD severity.
Our findings showed a negative association of sestrin levels and the coronary stenosis severity.
氧化应激参与冠状动脉疾病（CAD）的病理过程已得到充分证实。Sestrin蛋白家族最近成为氧化应激的重要抑制因子。然而，仅有少数研究报道了CAD患者中Sestrin蛋白的水平及其临床意义。
研究对象为90名因心脏血管造影术而转诊至心脏血管造影科的患者。32名受试者被诊断为稳定型CAD，20名患者患有不稳定型CAD，38名受试者无CAD。所有患者均接受了血管造影术，并通过改良的Gensini评分计算冠状动脉狭窄的严重程度。采用常规方法测定空腹血样中的葡萄糖、甘油三酯、总胆固醇、高密度脂蛋白、低密度脂蛋白、高敏C反应蛋白和血液学参数水平。通过酶联免疫吸附测定法（ELISA）检测血浆中Sestrin-1和Sestrin-2的水平。
尽管两个病例组的血浆Sestrin-1水平均显著低于对照组（P<0.001），但两组患者之间的Sestrin-1水平无显著差异。CAD组的Sestrin-2水平也显著低于对照组（P=0.001），但稳定型和不稳定型患者之间未发现显著差异。在整个研究对象中，血浆Sestrin-1和Sestrin-2与冠状动脉评分呈负相关。多因素分析显示，只有Sestrin-2水平与CAD严重程度显著相关。
我们的研究结果表明Sestrin蛋白水平与冠状动脉狭窄严重程度呈负相关。
