Lejri Imane, Vukalović Ina, Grimm Amandine, Eckert Anne
Neurobiology Lab for Brain Aging and Mental Health, University Psychiatric Clinics UPK, Basel, Switzerland.
Research Cluster Molecular and Cognitive Neurosciences, University of Basel, Basel, Switzerland.
Front Pharmacol. 2025 Jun 12;16:1495997. doi: 10.3389/fphar.2025.1495997. eCollection 2025.
EGb 761 is a proprietary extract from leaves approved as an herbal medication for the treatment of dementia and its related disorders. Preclinical studies highlight antioxidant, ROS scavenging, mitochondria-stabilizing, and neuroplastic properties as some of the reported pharmacological activities. Efficacy is traditionally ascribed to terpene lactones and flavone glycosides. However, these quantified known active compounds in EGb 761 only cover approximately 30% of the mass balance, and there is the possibility that additional compounds from the residual 70% may enhance the activity of the quantified extract EGb 761. Proanthocyanidins (PACs) are a quantitatively relevant component in EGb 761, and some pharmacological activity has been reported for PACs from Ginkgo and other herbal sources. In this study, we focused on the effects of EGb 761 and its isolated PACs on mitochondrial bioenergetics and neuroplasticity in the human neuroblastoma cell line SH-SY5Y. We successfully demonstrated positive effects of EGb 761 and its isolated PACs on several mitochondrial characteristics and neurite outgrowth. As PACs exhibited similar effects compared to the respective extract concentration, they can be considered a pharmacologically relevant component of EGb 761.
EGb 761是一种从银杏叶中提取的专利提取物，被批准作为一种草药药物用于治疗痴呆及其相关疾病。临床前研究强调了抗氧化、清除活性氧、稳定线粒体和神经可塑性等一些已报道的药理活性。传统上认为其疗效归因于萜类内酯和黄酮糖苷。然而，EGb 761中这些已定量的已知活性化合物仅占质量平衡的约30%，并且剩余70%中的其他化合物有可能增强已定量提取物EGb 761的活性。原花青素（PACs）是EGb 761中一个在数量上相关的成分，并且已经报道了来自银杏和其他草药来源的PACs具有一些药理活性。在本研究中，我们聚焦于EGb 761及其分离出的PACs对人神经母细胞瘤细胞系SH-SY5Y中线粒体生物能量学和神经可塑性的影响。我们成功证明了EGb 761及其分离出的PACs对几种线粒体特征和神经突生长具有积极作用。由于PACs与相应提取物浓度相比表现出相似的效果，它们可被视为EGb 761的一种具有药理相关性的成分。
