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使用PANDORA-seq技术对子宫内膜异位症中tRNA衍生片段的表达进行综合分析。

Comprehensive analysis of tRNA-derived fragment expression in endometriosis using PANDORA-seq technology.

作者信息

Huang Jingyao, Cheng Junping, Zhang Dandan, Wang Ying, Liang Huanying, Liu Liling

机构信息

Reproductive Medical and Genetic Center, Academy of Medical Sciences of Guangxi Autonomous Region, People's Hospital of Guangxi Autonomous Region, Nanning, China.

Graduate College of Youjiang Medical University for Nationalities, Baise, China.

出版信息

RNA Biol. 2025 Jun 27;22(1):1-14. doi: 10.1080/15476286.2025.2525707.

DOI:10.1080/15476286.2025.2525707
PMID:40575905
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12233860/
Abstract

BACKGROUND

Endometriosis is a common gynaecological disease and there is no reliable non-invasive biomarker for its unknown pathogenesis. TRF is differentially expressed in a variety of cancers and is a new non-invasive biomarker. The aim of this study was to reveal the full landscape of tRF expression profile in endometriosis using PANDORA-seq, which will provide strong target support for early diagnosis and treatment.

METHODS

PANDORA-seq was used to detect the eutopic and ectopic endometrial tissues of 4 patients with ovarian endometriosis and 4 normal endometrial tissues in the control group, and qRT-PCR was performed to verify. The target genes of DEtRF were predicted by TargetScan and miRanda, and the potential functions of differential tRFs were studied by bioinformatics such as Gene Ontology (GO) and Kycrto Encyclopedia of Genes and Genomes (KEGG), so as to further elucidate the pathogenesis of endometriosis.

RESULTS

Under the screening conditions of |Fold Change| ≥ 2 and Padj < 0.05, a total of 13 common differentially expressed tRFs were identified when comparing the disease groups, defined as endometriosis-affected eutopic endometrial tissue (EU) and ectopic endometrial tissue (EC), with the control group consisting of eutopic endometrial tissue from normal uteri (EN). Eleven DEtRFs target genes were highly enriched in endometriosis-related signalling pathways, such as MAPK, Ras, p53 and mitophagy-related pathways.

CONCLUSION

Differentially expressed tRF may be involved in the development of endometriosis by regulating target genes in MAPK and autophagy signalling pathways. DEtRF is expected to be a new non-invasive biomarker for endometriosis.

摘要

背景

子宫内膜异位症是一种常见的妇科疾病，其发病机制不明，尚无可靠的非侵入性生物标志物。tRF在多种癌症中存在差异表达，是一种新的非侵入性生物标志物。本研究旨在利用PANDORA-seq揭示子宫内膜异位症中tRF表达谱的全貌，为早期诊断和治疗提供有力的靶点支持。

方法

采用PANDORA-seq检测4例卵巢子宫内膜异位症患者的在位和异位子宫内膜组织以及对照组4例正常子宫内膜组织，并进行qRT-PCR验证。通过TargetScan和miRanda预测DEtRF的靶基因，利用基因本体论（GO）和京都基因与基因组百科全书（KEGG）等生物信息学方法研究差异tRF的潜在功能，以进一步阐明子宫内膜异位症的发病机制。

结果

在|倍数变化|≥2且Padj＜0.05的筛选条件下，将疾病组（定义为受子宫内膜异位症影响的在位子宫内膜组织（EU）和异位子宫内膜组织（EC））与由正常子宫的在位子宫内膜组织组成的对照组（EN）进行比较时，共鉴定出13个常见的差异表达tRF。11个DEtRF的靶基因在子宫内膜异位症相关信号通路中高度富集，如MAPK、Ras、p53和线粒体自噬相关通路。

结论

差异表达的tRF可能通过调节MAPK和自噬信号通路中的靶基因参与子宫内膜异位症的发生发展。DEtRF有望成为子宫内膜异位症新的非侵入性生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/7e70927e6591/KRNB_A_2525707_F0010b_OC.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/be68e8201d6c/KRNB_A_2525707_F0005_OC.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/fc290f109151/KRNB_A_2525707_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/e274660fd1e2/KRNB_A_2525707_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/fdaf908d766f/KRNB_A_2525707_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/a20ab00bb611/KRNB_A_2525707_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/be68e8201d6c/KRNB_A_2525707_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/1023c5ddd082/KRNB_A_2525707_F0006_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/48c06206c478/KRNB_A_2525707_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/6edc6896be0f/KRNB_A_2525707_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/e8dbae61abb3/KRNB_A_2525707_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/5aa9752b6def/KRNB_A_2525707_F0010a_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/18cd/12233860/7e70927e6591/KRNB_A_2525707_F0010b_OC.jpg

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