Rasadi Arezoo, Talesh Sasani Soheila, Ajamian Farzam
Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran.
Mol Neurobiol. 2025 Jun 27. doi: 10.1007/s12035-025-05159-y.
BACE1 promotes aggregation of β-amyloid in Alzheimer's patients' brains. MicroRNAs play roles in gene expression regulation. Furthermore, BACE1 is a target for microRNAs. There is a crosstalk between the peripheral and central immune systems in the etiology of Alzheimer's disease. This study investigates the alterations in expression of BACE1, along with miR-15a-5p and miR-19b-3p genes, in peripheral blood mononuclear cells (PBMCs) of late-onset Alzheimer's disease (LOAD) patients. The levels of BACE1 mRNA, miR-15a-5p, and miR-19b-3p were measured in PBMCs using a real-time quantitative PCR method. Cytoscape software was used to identify the putative target genes of these microRNAs. Significant increase in BACE1 levels (mean ± SD: 2.076 ± 0.5308), and decrease in both miR-15a-5p and miR-19b-3p expressions (0.3656 ± 0.1056 and 0.7296 ± 0.1933, respectively) were observed. Altered BACE1, miR-15a-5p, and miR-19b-3p expressions suggest novel indicators for early LOAD diagnosis. We illustrated target genes by which these microRNAs may regulate BACE1 expression.
β-分泌酶1（BACE1）促进阿尔茨海默病患者大脑中β-淀粉样蛋白的聚集。微小RNA在基因表达调控中发挥作用。此外，BACE1是微小RNA的一个作用靶点。在阿尔茨海默病的病因学中，外周和中枢免疫系统之间存在相互作用。本研究调查晚发型阿尔茨海默病（LOAD）患者外周血单个核细胞（PBMCs）中BACE1以及miR-15a-5p和miR-19b-3p基因表达的变化。采用实时定量PCR方法检测PBMCs中BACE1 mRNA、miR-15a-5p和miR-19b-3p的水平。使用Cytoscape软件鉴定这些微小RNA的潜在靶基因。观察到BACE1水平显著升高（平均值±标准差：2.076±0.5308），miR-15a-5p和miR-19b-3p表达均降低（分别为0.3656±0.1056和0.7296±0.1933）。BACE1、miR-15a-5p和miR-19b-3p表达的改变提示了早期LOAD诊断的新指标。我们阐明了这些微小RNA可能调控BACE1表达的靶基因。
