The First Department of Neurology, Rizhao People's Hospital, Rizhao, 276800, Shandong, China.
Department of Neurology, Zhucheng People's Hospital, Zhucheng, 262200, Shandong, China.
Neurosci Lett. 2021 Jun 21;756:135953. doi: 10.1016/j.neulet.2021.135953. Epub 2021 May 9.
The current study investigated the expression change and clinical value of miR-148a-3p in AD patients, and further examined the role of miR-148a-3p in Aβ-induced neurotoxicity in SH-SY5Y cells.
qRT-PCR was used for the measurement of miR-148a-3p expression levels. ROC curve was established to calculate the diagnostic value of serum miR-148a-3p for AD. CCK-8 and flow cytometry assay was applied for the detection of cell viability and apoptosis. Additionally, the luciferase reporter assay was performed to confirm the target relationship between ROCK1 and miR-148a-3p.
Serum miR-148a-3p was downregulated in AD patients compared with that in healthy controls, and was positively associated with the MMSE score in AD patients. Serum miR-148a-3p had the potential to distinguish AD patients from healthy controls, and the diagnostic sensitivity and specificity were respectively 85.5 % and 87.0 % at a cutoff value of 0.827. MiR-148a-3p attenuated Aβ25-35 induced neurotoxicity in SH-SY5Y cells, and ROCK1 was the target gene.
Serum miR-148a-3p is correlated with MMSE score in AD patients, and it might be helpful for the AD diagnosis. Overexpression of miR-148a-3p attenuated Aβ induced neurotoxicity in AD by targeting ROCK1.
本研究旨在探讨 miR-148a-3p 在 AD 患者中的表达变化及其临床价值，并进一步研究 miR-148a-3p 在 Aβ 诱导的 SH-SY5Y 细胞神经毒性中的作用。
采用 qRT-PCR 检测 miR-148a-3p 的表达水平。绘制 ROC 曲线，计算血清 miR-148a-3p 对 AD 的诊断价值。CCK-8 和流式细胞术检测细胞活力和凋亡。此外，还进行了荧光素酶报告基因实验来验证 ROCK1 与 miR-148a-3p 之间的靶关系。
与健康对照组相比，AD 患者血清 miR-148a-3p 表达下调，且与 AD 患者 MMSE 评分呈正相关。血清 miR-148a-3p 具有区分 AD 患者与健康对照组的潜力，截断值为 0.827 时，诊断的敏感性和特异性分别为 85.5%和 87.0%。miR-148a-3p 可减轻 Aβ25-35 诱导的 SH-SY5Y 细胞神经毒性，ROCK1 是其靶基因。
血清 miR-148a-3p 与 AD 患者的 MMSE 评分相关，可能有助于 AD 的诊断。过表达 miR-148a-3p 通过靶向 ROCK1 减轻 Aβ 诱导的 AD 神经毒性。
