Abdul-Majeed Zaid Mahmood, Al-Atrakji Mohammed Qasim Yahya Malallah A, Ridha-Salman Hayder
Department of Pharmacology, College of Medicine, University of Baghdad, Baghdad, Iraq.
Al-Mustaqbal University, College of Pharmacy, 51001 Hillah, Baghdad, Iraq.
J Mol Histol. 2025 Jun 27;56(4):206. doi: 10.1007/s10735-025-10438-y.
Indomethacin (IND) belongs to nonsteroidal anti-inflammatory drugs (NSAIDs) prescribed for the treatment of rheumatoid diseases and linked to the development of gastric ulcers in many people. Cranberry is a rich source of polyphenols and flavonoids, which have powerful antioxidant and anti-inflammatory properties. The study aimed to evaluate the activity of cranberry aqueous extract on IND-induced gastric ulcers in albino rats. Twenty adult male rats were sequentially assigned to four groups of five each. The control group consumes distilled water (DW) orally for 15 days. The IND group received a single oral dosage (60 mg/kg) of IND. The omeprazole (OMP) group got 60 mg/kg of IND as a single oral dose and then 20 mg/kg/day of OMP for 15 days. The cranberry group was given a single dose of IND 60 mg/kg orally and 200 mg/kg/day of cranberry aqueous extract for 15 days. Rats were euthanized on day 16, and gastric tissues were removed for biochemical and histopathological evaluations. Cranberry extract considerably ameliorated the severity of IND-induced gastric ulcerations and fixed the histopathological alterations, including mucosal membrane necrosis, congestion, inflammatory cell penetration, and deteriorations of GIT. Furthermore, IND-exposed rats treated with cranberry extract exhibited dramatically lower serum levels of oxidative biomarkers like MDA and inflammatory biomarkers like TNF-α and IL-6, but higher levels of anti-oxidative biomarkers like SOD and GPx and anti-inflammatory biomarkers like IL-10. The bioactive flavonoids and polyphenols content of cranberry extract could account for its profound gastroprotective effects. The anti-oxidative and anti-inflammatory properties of cranberry extract could be a promising strategy for ameliorating the IND-aggravated gastrotoxicity.
吲哚美辛（IND）属于非甾体抗炎药（NSAIDs），用于治疗类风湿疾病，但在许多人身上与胃溃疡的发生有关。蔓越莓富含多酚和黄酮类化合物，具有强大的抗氧化和抗炎特性。本研究旨在评估蔓越莓水提取物对吲哚美辛诱导的白化大鼠胃溃疡的活性。将20只成年雄性大鼠依次分为四组，每组五只。对照组口服蒸馏水（DW）15天。吲哚美辛组接受单次口服剂量（60毫克/千克）的吲哚美辛。奥美拉唑（OMP）组单次口服60毫克/千克的吲哚美辛，然后连续15天每天口服20毫克/千克的奥美拉唑。蔓越莓组口服单次剂量60毫克/千克的吲哚美辛，并连续15天每天口服200毫克/千克的蔓越莓水提取物。在第16天对大鼠实施安乐死，并取出胃组织进行生化和组织病理学评估。蔓越莓提取物显著改善了吲哚美辛诱导的胃溃疡的严重程度，并修复了组织病理学改变，包括黏膜膜坏死、充血、炎症细胞浸润以及胃肠道的恶化。此外，用蔓越莓提取物治疗的吲哚美辛暴露大鼠的血清氧化生物标志物如丙二醛（MDA）和炎症生物标志物如肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平显著降低，但抗氧化生物标志物如超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）以及抗炎生物标志物如白细胞介素-10（IL-10）水平较高。蔓越莓提取物中的生物活性黄酮类化合物和多酚含量可以解释其深远的胃保护作用。蔓越莓提取物的抗氧化和抗炎特性可能是改善吲哚美辛加重的胃毒性的一种有前景的策略。
