Conceição-Santos A L, Velarde J M D S, Joaquim D C, Figueiredo J R
Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Department of Animal Science, Center of Agrarian Sciences, Federal University of Sergipe, São Cristovão, SE, Brazil.
Theriogenology. 2025 Oct 15;246:117557. doi: 10.1016/j.theriogenology.2025.117557. Epub 2025 Jun 24.
This study aimed to evaluate the effects of trans-anethole supplementation on in vitro reproductive outcomes using a meta-analysis approach. A total of 128 records were retrieved from four major scientific databases. After screening and eligibility assessment, 6 studies were included in the final analysis. These studies reported outcomes from in vitro follicle culture, oocyte in vitro maturation, and embryo culture across multiple species and varying concentrations of trans-anethole. Relative risks (RR) and 95 % confidence intervals (CI) were calculated, and forest plots were used for visual synthesis. Risk of bias was assessed using SYRCLE's tool. The results demonstrated a dose-dependent and species-specific effect of trans-anethole. Moderate concentrations (30-300 μg/mL) improved oocyte maturation (MII), cleavage, and blastocyst rates-particularly in goats, where MII rates increased by up to 25.8 %. In cattle, outcomes were inconsistent, with some studies reporting benefits and others showing negative effects at high doses (≥2000 μg/mL). Cleavage and embryo development followed a similar dose-response pattern. Low concentrations generally improved cleavage and blastocyst rates, while higher concentrations reduced performance, especially in goats. Significant heterogeneity (I = 73.7-100 %) was observed across studies, highlighting the influence of species, treatment stage, and concentration. Overall, trans-anethole shows promise as a supplement in ART systems, particularly at low to moderate concentrations. However, its efficacy is highly context-dependent. Further research with standardized protocols is needed to define optimal dosing strategies and clarify its mechanisms of action in reproductive biotechnology.
本研究旨在采用荟萃分析方法评估反式茴香脑补充剂对体外生殖结果的影响。从四个主要科学数据库中检索到共128条记录。经过筛选和合格性评估，最终分析纳入了6项研究。这些研究报告了跨多个物种以及不同浓度反式茴香脑的体外卵泡培养、卵母细胞体外成熟和胚胎培养的结果。计算了相对风险（RR）和95%置信区间（CI），并使用森林图进行直观综合分析。使用SYRCLE工具评估偏倚风险。结果表明反式茴香脑具有剂量依赖性和物种特异性效应。中等浓度（30 - 300μg/mL）可提高卵母细胞成熟率（MII）、卵裂率和囊胚率，尤其是在山羊中，MII率提高了25.8%。在牛中，结果不一致，一些研究报告了益处，而另一些研究表明高剂量（≥2000μg/mL）时有负面影响。卵裂和胚胎发育遵循类似的剂量反应模式。低浓度通常可提高卵裂率和囊胚率，而高浓度则降低性能，尤其是在山羊中。研究之间观察到显著的异质性（I = 73.7 - 100%），突出了物种、处理阶段和浓度的影响。总体而言，反式茴香脑有望作为辅助生殖技术系统中的补充剂，特别是在低至中等浓度时。然而，其疗效高度依赖于具体情况。需要采用标准化方案进行进一步研究，以确定最佳给药策略并阐明其在生殖生物技术中的作用机制。
