使用天然低共熔溶剂的绿色超声辅助萃取法的多变量优化，用于通过火焰原子吸收光谱法测定速溶咖啡样品中的铁、锰和锌。
A multivariate optimization of a green ultrasound-assisted extraction method using a natural deep eutectic solvent for the determination of Fe, Mn, and Zn in instant coffee samples by F AAS.
作者信息
Costa Giovanna C, Soares Luana M, Junior João B P, Araujo Rennan Geovanny O, Cunha Francisco A S
机构信息
Universidade Federal da Bahia, Instituto de Química, Campus de Ondina, 40170-115, Salvador, BA, Brazil.
Departamento de Ciências Exatas, Universidade Estadual de Feira de Santana, 44036-900, Feira de Santana, BA, Brazil.
出版信息
Food Chem. 2025 Nov 1;491:145271. doi: 10.1016/j.foodchem.2025.145271. Epub 2025 Jun 25.
This study presents the development of a green ultrasound-assisted extraction (UAE) method using a natural deep eutectic solvent (NADES) composed of malic acid and citric acid for the determination of Fe, Mn, and Zn in instant coffee samples by flame atomic absorption spectrometry (F AAS). The solvent ratio was previously optimized using a combination of factorial and Box-Behnken designs. A two-level full factorial design (2) was employed to optimize the NADES-UAE method. Extraction was performed using 0.3 g of coffee, 5.0 mL of NADES, and 35 min of sonication. The method achieved detection limits of 0.26, 0.55, and 3.38 μg g, for Zn, Mn, and Fe, respectively. Method accuracy was assessed using a certified reference material of green coffee, yielding recoveries ranging from 92.4 % to 112.3 %. The method was successfully applied to instant coffee samples, with metal concentrations ranging from 2.14 μg g (Mn) to 60.44 μg g (Fe).
本研究提出了一种绿色超声辅助萃取（UAE）方法，该方法使用由苹果酸和柠檬酸组成的天然低共熔溶剂（NADES），通过火焰原子吸收光谱法（F AAS）测定速溶咖啡样品中的铁、锰和锌。先前使用析因设计和Box-Behnken设计的组合对溶剂比例进行了优化。采用二级全因子设计（2）优化NADES-UAE方法。使用0.3 g咖啡、5.0 mL NADES进行萃取，并超声处理35分钟。该方法对锌、锰和铁的检出限分别为0.26、0.55和3.38 μg g。使用生咖啡豆标准参考物质评估方法的准确性，回收率在92.4%至112.3%之间。该方法成功应用于速溶咖啡样品，金属浓度范围为2.14 μg g（锰）至60.44 μg g（铁）。
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