Ding Qianqian, He Yifan, Gao Weifeng, Gao Xiaojian, Zhang Yao, Zhang Xiaojun, Jiang Qun
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Comp Biochem Physiol A Mol Integr Physiol. 2025 Sep;307:111901. doi: 10.1016/j.cbpa.2025.111901. Epub 2025 Jun 27.
Insulin-like growth factor binding proteins (IGFBPs) are key regulators of insulin-like growth factors (IGFs), which play essential roles in growth and development. In this study, a IGFBP related protein cDNA from Macrobrachium rosenbergii (designated Mr-IGFBP-rP) was identified. The full-length cDNA of Mr-IGFBP-rP is 1494 base pairs (bp) including a 22 bp 5'-untranslated region (UTR), a 707 bp 3'-UTR, and a 765 bp open reading frame (ORF) encoding a 254-amino acid protein. The Mr-IGFBP-rP protein contains three conserved domains: an IGF-binding domain (amino acids 34-89), a Kazal-type serine protease inhibitor domain (102-125), and an immunoglobulin-like C2-type domain (147-245). Sequence analysis revealed that Mr-IGFBP-rP shares 75.74 % identity with the IGFBP-rP1 of Palaemon carinicauda and 63.26 % with that of Penaeus chinensis. Expression analysis showed that Mr-IGFBP-rP is most highly expressed in the abdominal ganglia, followed by gill, heart, and brain. Eleven single nucleotide polymorphisms (SNPs) were identified within Mr-IGFBP-rP. Of these, three SNPs showed significant associations with growth traits in M. rosenbergii. Specifically, individuals with the AA genotype at the 5263-G/A locus exhibited greater body weight. Those with the TT genotype at the 5302-G/T locus showed increased body weight, cephalothorax height, and abdominal segment 1 height. In contrast, the CC genotype at the 5743-G/C locus was associated with reduced cephalothorax length, cephalothorax height, abdominal segment 1 height, and abdominal length. This study lays a foundation for understanding the role of Mr-IGFBP-rP in growth regulation in M. rosenbergii. The identified SNPs hold potential as candidate markers for marker-assisted selection in this species.
胰岛素样生长因子结合蛋白（IGFBPs）是胰岛素样生长因子（IGFs）的关键调节因子，IGFs在生长和发育中起着至关重要的作用。在本研究中，鉴定出一种来自罗氏沼虾的IGFBP相关蛋白cDNA（命名为Mr-IGFBP-rP）。Mr-IGFBP-rP的全长cDNA为1494个碱基对（bp），包括一个22 bp的5'-非翻译区（UTR）、一个707 bp的3'-UTR和一个765 bp的开放阅读框（ORF），编码一个254个氨基酸的蛋白质。Mr-IGFBP-rP蛋白包含三个保守结构域：一个IGF结合结构域（第34-89位氨基酸）、一个Kazal型丝氨酸蛋白酶抑制剂结构域（102-125）和一个免疫球蛋白样C2型结构域（147-245）。序列分析表明，Mr-IGFBP-rP与脊尾白虾的IGFBP-rP1的同一性为75.74%，与中国对虾的同一性为63.26%。表达分析表明，Mr-IGFBP-rP在腹神经节中表达最高，其次是鳃、心脏和脑。在Mr-IGFBP-rP中鉴定出11个单核苷酸多态性（SNP）。其中，三个SNP与罗氏沼虾的生长性状显著相关。具体而言，在5263-G/A位点具有AA基因型的个体表现出更大的体重。在5302-G/T位点具有TT基因型的个体体重、头胸甲高度和第1腹节高度增加。相反，在5743-G/C位点的CC基因型与头胸甲长度、头胸甲高度、第1腹节高度和腹长的降低有关。本研究为了解Mr-IGFBP-rP在罗氏沼虾生长调节中的作用奠定了基础。所鉴定的SNP有潜力作为该物种标记辅助选择的候选标记。
