Zhao Junqi, Guan Zhiwei, Fan Shuhua, Qiu Jianli, Xu Yan, Zhao Qiong
Pediatric Hospital, First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou 450000, P.R. China.
School of Pediatrics, Henan University of Chinese Medicine, Zhengzhou 450046, P.R. China.
J Microbiol Biotechnol. 2025 Jun 23;35:e2412037. doi: 10.4014/jmb.2412.12037.
The hub metabolites and hub microorganisms that play an important role in the intervention of Qi-yin deficiency syndrome with diarrhea by Modified Renshen Wumei Decoction (MRWD) are still unclear. Therefore, we explored it based on multi-omics analysis. A total of 32 Sprague-Dawley rats were collected and randomly allocated into different groups. Subsequently, blood samples and fecal samples were collected from all 32 rats for non-targeted metabolome sequencing and 16S rRNA gene sequencing respectively. Among them, fecal samples of 24 rats were also used for targeted metabolomics sequencing (6 rats in each group). Pathological findings, and D-lactate, diamine oxidase (DAO), aquaporin 3 (AQP3), and aquaporin 8 (AQP8) levels in serum and colon samples were evaluated after the 21-day trial, and the results showed that diarrhea caused intestinal injury, which was ameliorated by infusion of MRWD. Based on multi-omics sequencing analysis, five microorganisms were superior to the positive control in MRWD intervention. Moreover, Eisenbergiella, Corynebacterium, and unidentified Oscillospiraceae exhibited significant discriminatory capabilities between groups C and D, groups B and C, as well as groups A and C; thus they were identified as hub microorganisms. On the other hand, significant differences in metabolites were observed among different groups with respect to the metabolome. These metabolites exhibited significant enrichment in metabolic pathways such as Butanoate metabolism, Propanoate metabolism, and Pyruvate metabolism. Further correlation analysis revealed that 2-Methylbutyrate was identified as a hub metabolites associated with blood and fecal microorganisms. Moreover, there were complex regulatory relationships between these hub microbes and hub metabolites.
加味人参乌梅汤（MRWD）干预气阴两虚型腹泻的核心代谢物和核心微生物仍不明确。因此，我们基于多组学分析对其进行了探索。共收集32只Sprague-Dawley大鼠，并随机分为不同组。随后，分别从所有32只大鼠中采集血样和粪便样本，进行非靶向代谢组测序和16S rRNA基因测序。其中，还选取24只大鼠的粪便样本进行靶向代谢组测序（每组6只）。21天试验后评估病理结果以及血清和结肠样本中D-乳酸、二胺氧化酶（DAO）、水通道蛋白3（AQP3）和水通道蛋白8（AQP8）的水平，结果显示腹泻导致肠道损伤，而MRWD灌胃可改善这种损伤。基于多组学测序分析，5种微生物在MRWD干预方面优于阳性对照。此外，艾氏菌属、棒状杆菌属和未鉴定的 Oscillospiraceae 在C组和D组、B组和C组以及A组和C组之间表现出显著的区分能力；因此它们被确定为核心微生物。另一方面，在代谢组方面，不同组之间的代谢物存在显著差异。这些代谢物在丁酸代谢、丙酸代谢和丙酮酸代谢等代谢途径中表现出显著富集。进一步的相关性分析表明，2-甲基丁酸被确定为与血液和粪便微生物相关的核心代谢物。此外，这些核心微生物与核心代谢物之间存在复杂的调控关系。
