Basile Giuseppe Stefano, Calcagno Damiano, Tuccitto Nunzio, Sbardella Diego, Grasso Giuseppe
Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125, Catania, Italy.
Scuola Superiore di Catania, University of Catania, Via Valdisavoia 9, 95123, Catania, Italy.
Chemphyschem. 2025 Aug 23;26(16):e202500138. doi: 10.1002/cphc.202500138. Epub 2025 Jun 29.
Information regarding the dimension and the shape of molecules in solution represents a holy grail for chemists. Recently, newly designed surface plasmon resonance (SPR) methods to precisely measure the diffusion coefficients (D) (D-SPR) have been developed and applied successfully to a variety of molecules, ranging from diverse long-chain alcohols to protein conformers and oligomers involved in conformational disorders, mostly represented by neurodegenerative processes of nervous tissues of the brain and retina. The dependence of D on the molecular size, shape, and oligomerization state of different molecules has been widely investigated, opening up new avenues and tools for chemical, biochemical, and clinical research. Herein, the historical basis and the development of the newly proposed D-SPR method are briefly described to obtain meaningful information about molecular features that are otherwise hard to characterize using more common and traditional bioanalytical approaches.
有关溶液中分子尺寸和形状的信息是化学家们梦寐以求的。最近，新设计的用于精确测量扩散系数（D）的表面等离子体共振（SPR）方法（D-SPR）已被开发出来，并成功应用于各种分子，从各种长链醇到参与构象紊乱的蛋白质构象异构体和寡聚体，这些紊乱大多以大脑和视网膜神经组织的神经退行性过程为代表。D对不同分子的大小、形状和寡聚化状态的依赖性已得到广泛研究，为化学、生物化学和临床研究开辟了新途径和新工具。在此，简要描述新提出的D-SPR方法的历史基础和发展，以获取有关分子特征的有意义信息，而这些信息用更常见和传统的生物分析方法很难表征。
