Lin Xueqi, Liu Shichu, Yuan Jiayue, Cong Jing, Feng Qianhong, Xie Wenyu, Yan Yufeng, Rao Yuliang, Feng Chenli, Zhou Hao, Dai Jinhui
From the Shanghai Institute of Infectious Disease and Biosecurity, Zhongshan Hospital, Fudan University, Shanghai, China (Lin, Dai); Department of Ophthalmology, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China (Lin, Liu, Yuan, Cong, Q. Feng, C. Feng, Dai); Bengbu Medical University, Bengbu, Anhui, China (Xie); Basic Medical Experimental Teaching Center, Fudan University, Shanghai, China (Yan, Rao); Department of Ophthalmology, Eye & ENT Hospital Affiliated to Fudan University, Shanghai, China (Zhou).
J Cataract Refract Surg. 2025 Oct 1;51(10):915-921. doi: 10.1097/j.jcrs.0000000000001731.
To evaluate the corneal crosslinking (CXL) efficacy of riboflavin (RF) at 5 different concentrations (0.1%, 0.25%, 0.5%, 0.75%, and 1.0%) and determine the optimal concentration for maximizing the cornea's biomechanical performance.
Department of Ophthalmology, Zhongshan Hospital, Fudan University.
Experimental study.
The corneal epithelium was removed from all eyes. In the first part, 0.1% to 1.0% RF solutions were applied to rabbit eyes for 30 minutes, with 20% dextran used as the vehicle for the RF solutions. After application, RF concentration in the cornea and aqueous humor were evaluated. In the second part, after applying different RF solutions, the corneas underwent CXL with 9 mW/cm 2 UV-A light for 10 minutes. Stress-strain curves, Young modulus, and resistance to enzymatic digestion were measured.
107 eyes were used in total. RF concentrations in the cornea were 447.57 ± 28.72 μg/g, 536.51 ± 19.59 μg/g, 885.89 ± 111.77 μg/g, 1280.46 ± 103.86 μg/g, and 1668.68 ± 89.02 μg/g from 0.1% to 1.0% RF solutions, respectively. After CXL, the corneal stress of the other 4 groups was 123%, 147%, 116%, and 69% relative to the 0.1% RF group at 10% strain. Similarly, after CXL with RF solutions at 5 concentrations, the residual corneal dry weights were 0.45 ± 0.08 mg, 0.53 ± 0.03 mg, 0.62 ± 0.07 mg, 0.50 ± 0.04 mg, and 0.35 ± 0.05 mg, respectively.
The 0.5% RF group achieved the optimal corneal biomechanical performance under controlled experimental conditions, with decreased efficiency as the RF concentration further increased.
评估5种不同浓度（0.1%、0.25%、0.5%、0.75%和1.0%）的核黄素（RF）的角膜交联（CXL）效果，并确定能使角膜生物力学性能最大化的最佳浓度。
复旦大学附属中山医院眼科。
实验研究。
去除所有眼睛的角膜上皮。在第一部分中，将0.1%至1.0%的RF溶液应用于兔眼30分钟，20%的右旋糖酐用作RF溶液的载体。应用后，评估角膜和房水中的RF浓度。在第二部分中，应用不同的RF溶液后，角膜接受9 mW/cm²的紫外线A光照射10分钟进行CXL。测量应力-应变曲线、杨氏模量和抗酶消化能力。
共使用了107只眼睛。从0.1%至1.0%的RF溶液中，角膜中的RF浓度分别为447.57±28.72μg/g、536.51±19.59μg/g、885.89±111.77μg/g、1280.46±103.86μg/g和1668.68±89.02μg/g。CXL后，在10%应变时，其他4组的角膜应力相对于0.1%RF组分别为123%、147%、116%和69%。同样，用5种浓度的RF溶液进行CXL后，残留角膜干重分别为0.45±0.08mg、0.53±0.03mg、0.62±0.07mg、0.50±0.04mg和0.35±0.05mg。
在可控的实验条件下，0.5%RF组实现了最佳的角膜生物力学性能，随着RF浓度进一步增加，效率降低。
