Wu Junyuan, Ohnuki Shinsuke, Teyssonnière Élie, Yasukawa Taishi, Masuo Naohisa, Schacherer Joseph, Ohya Yoshikazu
Mitsubishi Corporation Life Sciences Limited, Tokyo Takarazuka Building 14F, 1-1-3 Yurakucho, Chiyoda-ku, Tokyo 100-0006, Japan.
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg. FSB-101, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture 277-8562, Japan.
G3 (Bethesda). 2025 Sep 3;15(9). doi: 10.1093/g3journal/jkaf145.
Cyberlindnera jadinii is a nonmodel yeast species recognized for its robust growth under diverse stress conditions, making it a promising candidate for various industrial applications. Here, we performed genome sequencing for genetic analysis and high-throughput phenotypic assays to examine 20 wild strains, focusing on the diversity of their genome, morphology, and fitness traits. We found that C. jadinii comprises a mixed population of diploid and triploid cells, with the latter exhibiting significantly larger cell sizes than the former, suggesting that ploidy plays a crucial role in determining cell morphology. In contrast, fitness traits, including growth under 24 different conditions, were more closely associated with SNP-defined lineages than with ploidy. Cyberlindnera jadinii exhibited a genetic diversity (π = 18 × 10-3) four times higher than that of Saccharomyces cerevisiae, possibly reflecting genetic diversity shaped by history and environment. The heterologous expression of the CjFPS1 homolog in S. cerevisiae enhanced its growth on glycerol and acetate, underscoring its conserved metabolic function in carbon utilization. Overall, our findings not only reveal the rich genotypic and phenotypic diversity of C. jadinii but also provide new insights into its potential applications as a robust industrial yeast for diverse biotechnological processes.
季也蒙毕赤酵母是一种非模式酵母物种，因其在多种应激条件下的强劲生长而受到认可，这使其成为各种工业应用的有前途的候选者。在此，我们进行了基因组测序以进行遗传分析和高通量表型分析，以检测20个野生菌株，重点关注它们的基因组、形态和适应性特征的多样性。我们发现季也蒙毕赤酵母由二倍体细胞和三倍体细胞的混合群体组成，后者的细胞大小明显大于前者，这表明倍性在决定细胞形态方面起着关键作用。相比之下，包括在24种不同条件下生长的适应性特征，与单核苷酸多态性（SNP）定义的谱系的关联比与倍性的关联更为密切。季也蒙毕赤酵母的遗传多样性（π = 18 × 10-3）比酿酒酵母高四倍，这可能反映了由历史和环境塑造的遗传多样性。季也蒙毕赤酵母CjFPS1同源物在酿酒酵母中的异源表达增强了其在甘油和乙酸盐上的生长，突出了其在碳利用方面保守的代谢功能。总体而言，我们的研究结果不仅揭示了季也蒙毕赤酵母丰富的基因型和表型多样性，还为其作为一种强大的工业酵母在各种生物技术过程中的潜在应用提供了新的见解。
