Wang Su Han, Wang Jian Ping, Feng Cheng
College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei 071000, China.
College of Science, Hebei Agricultural University, Baoding, Hebei 071000, China.
Food Chem X. 2025 May 30;29:102603. doi: 10.1016/j.fochx.2025.102603. eCollection 2025 Jul.
The residues of fluoroquinolones in foods of animal origin posed many threats to the consumer's health. The aim of this study was to developed a magnetic molecularly imprinted microsphere and aggregation induced emission luminogen based semi-homogeneous method for determination of fluoroquinolones in milk. Results showed the limits of detection for determination of 20 fluoroquinolones in milk were in the range of 0.4-11 pg/mL. The sensitivities were increased for up to 4329 folds in comparison with fluorescein isothiocyanate labeled tracer. Due to the ultra-high sensitivity, the milk sample could be analyzed directly after a 100-fold dilution, and the recoveries from fluoroquinolones fortified blank milk samples were in the range of 69.68 %-97.19 %. The method performances were better than the previous fluorescent (immune)assays for fluoroquinolones. This is the first paper reporting the use of magnetic molecularly imprinted microsphere and aggregation induced emission luminogen for determination of veterinary drug in food sample.
动物源性食品中的氟喹诺酮类药物残留对消费者健康构成诸多威胁。本研究的目的是开发一种基于磁性分子印迹微球和聚集诱导发光体的半均相方法，用于测定牛奶中的氟喹诺酮类药物。结果表明，测定牛奶中20种氟喹诺酮类药物的检测限在0.4 - 11 pg/mL范围内。与异硫氰酸荧光素标记示踪剂相比，灵敏度提高了4329倍。由于超高灵敏度，牛奶样品经100倍稀释后可直接分析，氟喹诺酮类药物强化空白牛奶样品的回收率在69.68% - 97.19%范围内。该方法的性能优于以往用于氟喹诺酮类药物的荧光（免疫）分析方法。这是第一篇报道使用磁性分子印迹微球和聚集诱导发光体测定食品样品中兽药的论文。
