Yadoung Sumed, Ishimatsu Ryoichi, Xu Zhen-Lin, Sringarm Korawan, Pata Supansa, Thongkham Marninphan, Chantara Somporn, Pattarawarapan Mookda, Hongsibsong Surat
Environmental Science Program, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Motooka, Nishiku, Fukuoka 819-0395, Japan.
Antibiotics (Basel). 2022 Oct 29;11(11):1512. doi: 10.3390/antibiotics11111512.
Fluoroquinolones (FQs) are among the antibiotics whose widespread use in farm-raised animals results in potentially harmful residues in the end products. Additionally, most Thai farmers use antibiotics. Amoxicillin and enrofloxacin were commonly used by pig farms, and hens were given enrofloxacin to prevent immunization side effects. Moreover, antibiotic overuse has harmed food safety in the long term, and the use of low-dose antibiotics causes bacterial resistance. Herein, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was used to make a fast, easy, sensitive, and cost-effective method for monitoring FQs residues. After immunizing hens with mixed multi-hapten ciprofloxacin-bovine serum albumin (CPFX-BSA) with norfloxacin-bovine serum albumin (NFX-BSA), the IgY antibody purified from egg yolk was used for the detection of FQs residues in chicken and pork samples. The efficiency of the IgY antibody showed excellent sensitivity, with 50% inhibitory concentration (IC) of enrofloxacin at 0.05 µg/mL, far below the MRLs defined by the EU for muscle samples, which was not to exceed 100 µg/kg. The recovery range for chicken muscle samples spiked with ENFX concentrations of 1.00-0.01 µg/mL was 86.65-112.71%, similar to pork samples, which were 84.24-117.22.2%. This method has a lot of potential for analyzing fluoroquinolones in complex samples quickly, easily, and at a low cost on-site. The IgY-based ic ELISA was developed to detect ciprofloxacin (CPFX), norfloxacin (NFX), and enrofloxacin (ENFX) residues; it confirms that IgY could be a promising choice for the detection of antibiotic residues in food samples.
氟喹诺酮类药物（FQs）是一类抗生素，其在养殖动物中的广泛使用会导致最终产品中出现潜在有害残留。此外，大多数泰国农民使用抗生素。养猪场常用阿莫西林和恩诺沙星，母鸡被投喂恩诺沙星以预防免疫副作用。此外，抗生素的过度使用长期以来损害了食品安全，低剂量抗生素的使用会导致细菌耐药性。在此，采用间接竞争酶联免疫吸附测定法（icELISA）建立了一种快速、简便、灵敏且经济高效的监测FQs残留的方法。用环丙沙星 - 牛血清白蛋白（CPFX - BSA）与诺氟沙星 - 牛血清白蛋白（NFX - BSA）的混合多抗原对母鸡进行免疫后，从蛋黄中纯化的IgY抗体用于检测鸡肉和猪肉样品中的FQs残留。IgY抗体的效率显示出极佳的灵敏度，恩诺沙星的50%抑制浓度（IC）为0.05μg/mL，远低于欧盟规定的肌肉样品最大残留限量（MRLs），即不超过100μg/kg。添加浓度为1.00 - 0.01μg/mL恩诺沙星的鸡肉肌肉样品的回收率范围为86.65 - 112.71%，与猪肉样品相似，猪肉样品的回收率为84.24 - 117.22%。该方法在快速、简便且低成本地现场分析复杂样品中的氟喹诺酮类药物方面具有很大潜力。基于IgY的ic ELISA被开发用于检测环丙沙星（CPFX）、诺氟沙星（NFX）和恩诺沙星（ENFX）残留；这证实了IgY可能是检测食品样品中抗生素残留的一个有前景的选择。
