Katayama Shiori, Shiraishi Kosuke, Kaji Kanae, Kawabata Kazuya, Tamura Naoki, Tani Akio, Yurimoto Hiroya, Sakai Yasuyoshi
Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan.
Department of Anatomy and Histology, School of Medicine, Fukushima Medical University, Hikarigaoka 1, Fukushima 960-1295, Japan.
ISME Commun. 2025 May 29;5(1):ycaf092. doi: 10.1093/ismeco/ycaf092. eCollection 2025 Jan.
Pink-pigmented facultative methylotrophs (PPFMs) capable of growth on methanol are dominant and versatile phyllosphere bacteria that provide positive effects on plant growth through symbiosis. However, the spatial behavior of PPFMs on plant surfaces and its molecular basis are unknown. Here, we show that sp. strain OR01 inoculated onto red perilla seeds colonized across the entire plant surface in the phyllosphere concomitant with the plant growth. During its transmission, strain OR01 was found to be present on the entire leaf surface with a preference to sites around the periphery, vein, trichome, and stomata. We found that methanol-sensing chemoreceptor MtpA-dependent chemotaxis (methylotaxis; chemotaxis toward methanol) and flagellin protein FliC-dependent motility facilitated the bacterial entry into the stomatal cavity and their colonization in the phyllosphere.
能够在甲醇上生长的粉红色兼性甲基营养菌（PPFMs）是优势且多功能的叶际细菌，通过共生对植物生长产生积极影响。然而，PPFMs在植物表面的空间行为及其分子基础尚不清楚。在此，我们表明，接种到紫苏种子上的sp. 菌株OR01随着植物生长在叶际中定殖于整个植物表面。在其传播过程中，发现菌株OR01存在于整个叶片表面，偏好于叶片边缘、叶脉、毛状体和气孔周围的部位。我们发现，甲醇感应化学感受器MtpA依赖的趋化作用（甲基趋化作用；对甲醇的趋化作用）和鞭毛蛋白FliC依赖的运动性促进了细菌进入气孔腔并在叶际中定殖。
