White-nose syndrome (WNS), caused by the fungus (), has led to significant mortality and species endangerment in North America. Bats in eastern China, however, carry low loads of and do not exhibit disease, suggesting natural resistance. To explore potential defenses, we isolated and screened antagonistic bacteria from the wing membranes of for their ability to inhibit growth. We employed the plate delineation isolation method to obtain 74 single strains, which were then screened for antagonistic effects through contact and non-contact inhibition experiments. A total of 18 antagonistic strains were isolated. After sequencing and comparison with the NCBI database, we identified eight known species and three unidentified species of antagonistic bacteria: , , , , , , , , sp., sp., and sp. Seventeen isolated strains showed varying degrees of inhibition by contact, while five species, including , , , , and sp., also inhibited via non-contact mode. We utilized solid-phase microextraction coupled with GC-MS to obtain approximately 20 volatile compounds, all of which exhibited inhibitory effects on the growth of , including ketones, aldehydes, and sulfur ethers. Notably, 5 ppm of 1-undecene, dimethyl trisulphide, and 2-nonanone each independently inhibited growth. These findings suggest that the antagonistic strains and their VOCs might help protect bats from WNS. Understanding the interactions between and skin flora, along with their VOCs, may be crucial in mitigating bats' vulnerability to WNS and developing effective mitigation strategies in the future.