Chu Yujia, Wang Hui, Wang TianHui, Li Jingjing, Feng Lei, Wu Hui, Jiang Tinglei, Feng Jiang
College of Life Science Jilin Agricultural University Changchun China.
Jilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests Changchun China.
Ecol Evol. 2025 Jun 27;15(7):e71666. doi: 10.1002/ece3.71666. eCollection 2025 Jul.
Mammals rely on an intricate network of circadian clocks to regulate daily metabolic and physiological processes throughout the body. The liver, as a key peripheral organ, plays a crucial role in coordinating circadian rhythms and metabolic regulation. Bats, the only mammals capable of true flight, present unique nocturnal behaviors, which have garnered significant research interest. However, the daily metabolic fluctuations in bat liver and the underlying mechanisms of circadian regulation remain largely unexplored. To elucidate the metabolic foundations and rhythmic patterns in bat liver, a comparative LC-MS analysis was conducted to characterize the type, composition, and abundance of metabolites in the liver of Asian particolored bats at four distinct time points throughout a single day. This analysis identified a total of 7211 metabolites, with approximately 2.0% exhibiting significant rhythmicity, though with varying rhythmic patterns. Integrating these findings with RNA-Seq data revealed that differentially expressed genes and differentially accumulated metabolites were significantly enriched in two key pathways: the protein digestion and absorption pathway and the glutathione metabolism pathway. Notably, our results suggest that L-glutamate and L-tryptophan may play pivotal roles in the metabolic regulation of hepatic circadian rhythms, as indicated by metabolomics analysis. This study provides a systematic examination of the cyclic variations in metabolites within the bat liver, uncovering the physiological and biochemical processes involved and thereby enhancing our understanding of the molecular mechanisms governing circadian rhythms in bats.
哺乳动物依靠复杂的生物钟网络来调节全身的日常代谢和生理过程。肝脏作为关键的外周器官，在协调昼夜节律和代谢调节方面发挥着至关重要的作用。蝙蝠是唯一能够真正飞行的哺乳动物，具有独特的夜间行为，这引起了大量研究兴趣。然而，蝙蝠肝脏的每日代谢波动以及昼夜调节的潜在机制在很大程度上仍未被探索。为了阐明蝙蝠肝脏的代谢基础和节律模式，进行了一项比较液相色谱-质谱分析，以表征单色菊头蝠肝脏在一天中四个不同时间点的代谢物类型、组成和丰度。该分析共鉴定出7211种代谢物，其中约2.0%表现出显著的节律性，不过节律模式各异。将这些发现与RNA测序数据相结合表明，差异表达基因和差异积累的代谢物在两个关键途径中显著富集：蛋白质消化和吸收途径以及谷胱甘肽代谢途径。值得注意的是，我们的结果表明，代谢组学分析表明，L-谷氨酸和L-色氨酸可能在肝脏昼夜节律的代谢调节中起关键作用。这项研究对蝙蝠肝脏内代谢物的循环变化进行了系统研究，揭示了其中涉及的生理和生化过程，从而增进了我们对蝙蝠昼夜节律调控分子机制的理解。
