Wang Yuming, Zhang Hu, Cao Ning, Qi Bingqian, Zhao Feng, Xie Jingjing
State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Transl Anim Sci. 2025 Jun 14;9:txaf035. doi: 10.1093/tas/txaf035. eCollection 2025.
The objective of this study was to evaluate the effect of coating on the recovery rate () of phytase activity during the steam conditioning-pelleting () process. A split-plot design was employed, with phytases assigned to the main plot and two conditioning temperatures (75 or 85 °C) assigned to the subplot. The whole plot was repeated four times. In Exp. 1, six phytases were analyzed, including an uncoated phytase () NP1 (NP1), two coated phytases () CP1 and CP2 derived from NP1, and three commercial phytases ( MP1-MP3. In Exp. 2, coating technology was refined based on the results of Exp. 1, and nine phytases were analyzed, including an NP2, five coated phytases CP3-CP7 derived from NP2, and three commercial phytases MP4-MP6. Phytase activity after the steam-conditioning, pelleting, and cooling process was analyzed, and the RR of phytase activity was calculated for each process. In Exp. 1, significant interactions between phytase and conditioning temperature on the RR of phytase activity were observed (< 0.05). The RR of CP1 and CP2 did not differ from that of NP1. Commercial phytase MP3 exhibited a lower RR than the other four phytases when conditioned at 75 °C (< 0.05). Except for MP3, the RR of phytases decreased as the conditioning temperature increased (< 0.05). In Exp. 2, the RR of phytase decreased as the conditioning temperature increased from 75 to 85 °C (< 0.05). Compared with NP2, the RR increased, and the loss rate of activity for all five coated phytase (CP3-CP7) decreased after the conditioning process (< 0.05). Commercial phytase MP4 and MP6 had comparable RR to NP2, while MP5 exhibited a comparable RR to CP3-CP7. In conclusion, the coating technology used in Exp. 1 did not increase the RR of phytase during the pelleting process, whereas the improved coating process employed in Exp. 2 effectively increased the thermostability of phytase.
本研究的目的是评估包被对植酸酶活性在调质制粒过程中的回收率（RR）的影响。采用裂区设计，将植酸酶分配到主区，两个调质温度（75或85℃）分配到副区。整个区组重复4次。在实验1中，分析了6种植酸酶，包括一种未包被的植酸酶（NP1）、两种由NP1衍生的包被植酸酶（CP1和CP2）以及三种商业植酸酶（MP1 - MP3）。在实验2中，根据实验1的结果对包被技术进行了优化，分析了9种植酸酶，包括一种NP2、五种由NP2衍生的包被植酸酶（CP3 - CP7）以及三种商业植酸酶（MP4 - MP6）。分析了调质、制粒和冷却过程后的植酸酶活性，并计算了每个过程中植酸酶活性的RR。在实验1中，观察到植酸酶与调质温度对植酸酶活性RR存在显著交互作用（P < 0.05）。CP1和CP2的RR与NP1的RR无差异。商业植酸酶MP3在75℃调质时的RR低于其他四种植酸酶（P < 0.05）。除MP3外，植酸酶的RR随调质温度升高而降低（P < 0.05）。在实验2中，当调质温度从75℃升高到85℃时，植酸酶的RR降低（P < 0.05）。与NP2相比，调质后所有五种包被植酸酶（CP3 - CP7）的RR升高，活性损失率降低（P < 0.05）。商业植酸酶MP4和MP6的RR与NP2相当，而MP5的RR与CP3 - CP7相当。总之，实验1中使用的包被技术在制粒过程中未提高植酸酶的RR，而实验2中采用的改进包被工艺有效提高了植酸酶的热稳定性。
