Akturk Ergun, Pinto Graça, Ostyn Lisa, Crabbé Aurélie, Melo Luís D R, Azeredo Joana, Coenye Tom
CEB - Centre of Biological Engineering, University of Minho, Braga, Portugal.
LABBELS - Associate Laboratory, Braga, Portugal.
Biofilm. 2024 Dec 18;9:100245. doi: 10.1016/j.bioflm.2024.100245. eCollection 2025 Jun.
and are opportunistic pathogens commonly found in biofilm-associated polymicrobial respiratory infections that are challenging to control. Studies performed in laboratory standard conditions suggest that bacterio(phages) and antibiotic combinations are more active against bacterial communities and biofilms than each agent alone. The purpose of this work was to study the antibacterial efficacy of phage-antibiotic combinations using an -like three-dimensional lung epithelial model that mimics aspects of the parental tissue, colonized by a mixed bacterial community of and . The bacterial population was targeted by phages specific to and/or gentamicin and ciprofloxacin. The results showed that was eradicated from the dual-species community when phage treatment was followed by gentamicin and was significantly reduced when followed by ciprofloxacin. Moreover, applying phages first followed by antibiotics demonstrated superior antibacterial activity compared to simultaneous treatment or treatments with the reverse order of application. This approach also reduced the population but not as significant as the population. Using an -like model we demonstrated that phages and antibiotics are effective against dual-species bacterial communities, particularly targeting the population. However, but the sequence in which these antimicrobials are applied significantly in fluences the effectiveness of bacterial killing.
和是常见于生物膜相关的多微生物呼吸道感染中的机会致病菌，难以控制。在实验室标准条件下进行的研究表明，噬菌体和抗生素组合对细菌群落和生物膜的活性比单独使用每种药物更高。这项工作的目的是使用一种类似的三维肺上皮模型来研究噬菌体 - 抗生素组合的抗菌效果，该模型模拟了亲代组织的某些方面，并被和的混合细菌群落定殖。细菌群体被特异性针对和/或庆大霉素和环丙沙星的噬菌体靶向。结果表明，当噬菌体治疗后使用庆大霉素时，双物种群落中的被根除，当使用环丙沙星时显著减少。此外，与同时治疗或以相反顺序应用治疗相比，先应用噬菌体再应用抗生素显示出更好的抗菌活性。这种方法也减少了群体，但不如群体减少显著。使用类似的模型，我们证明噬菌体和抗生素对双物种细菌群落有效，特别是针对群体。然而，这些抗菌剂的应用顺序显著影响细菌杀灭的有效性。
