加纳当代宫颈疾病患者队列中人类乳头瘤病毒18型（HPV-18）和45型（HPV-45）特异性变体的分析

Analysis of Human Papillomavirus-HPV-18 and HPV-45 Type-specific variants in a contemporary cohort of individuals with cervical disease in Ghana.

作者信息

Kaba Gladys, Stevenson Andrew, Sakyi Samuel A, Konney Thomas O, Titiloye Nicholas A, Oppong Samuel A, Amo-Antwi Kwabena, Agyemang-Yeboah Francis, Cuschieri Kate, Graham Sheila V

机构信息

Department of Molecular Medicine, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana, West Africa.

Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, PMB 31 Ho, Volta Region, Ghana, West Africa.

出版信息

Ghana Med J. 2024 Dec;58(4):311-321. doi: 10.4314/gmj.v58i4.9.

DOI:10.4314/gmj.v58i4.9

PMID:40585519

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12203745/

Abstract

OBJECTIVES

E6 and E7 DNA sequence of HPV-18 and HPV-45 lineage/sublineage variants in Ghana.

DESIGN

A cross-sectional study.

SETTING

Obstetrics/Gynaecology Directorate, Komfo Anokye Teaching Hospital, Kumasi and Department of Radiotherapy/Nuclear Medicine and the Family Planning Unit, Korle-Bu Teaching Hospital, Accra.

PARTICIPANTS

207 individuals referred with clinical suspicion of cervical cancer (CxCa) or confirmed CxCa/precancer cases.

METHODS

Cervical swabs were collected (from October 2018 to November 2020) from individuals, with L1 DNA positivity for HPV-18(40 samples) and/or HPV-45(28 samples), out of 207 samples tested for 24-HPV-genotypes. DNA was extracted from a convenience sample and HPV-E6/E7-PCR (33/40-HPV-18-+ve- or 20/28-HPV-45-+ve samples), sequencing, and BLAST analysis was carried out.

RESULTS

After PCR amplification, the E6/E7 gene regions of 26 out of 33(HPV-18+ve) samples and out of 20 (HPV-45+ve) samples were eligible for sequencing. For HPV-18 variants, 24 out of 26 samples (92.31%) were of lineage-B/C, including samples of lineage-C and 22 samples of lineage-B (out of which samples were with E7-SNP-C665T). Nine out of ten HPV-45 variants were sublineage-A1, of which samples harboured both E6-SNPs-C134T and C4I7T including one sample with E6-SNPs-C134T, G415C, C4I7T detected together.

CONCLUSIONS

Our study confirms a dominance of HPV-45-sublineage-A1 and HPV-18-lineage-B (with rare occurrence of Africa-specific HPV-18-lineage-C) variants in Ghana. Our study provides preliminary data on E6/E7 SNPs of HPV-18 and HPV-45 lineages and sublineages among CxCa cases in Ghana. We hope our data will inform future studies on pattern and distribution of HPV type-specific nucleotide changes that can be useful for therapeutic intervention.

FUNDING

UK Government, (GK) Commonwealth Split-Site Scholarship with research support grant, number GHCN201823. GK also received Ghana public universities senior members book & research allowance, for research activities carried out in Ghana.

摘要

目的

加纳HPV - 18和HPV - 45谱系/亚谱系变体的E6和E7 DNA序列。

设计

横断面研究。

地点

库马西Komfo Anokye教学医院妇产科、阿克拉Korle - Bu教学医院放疗/核医学科及计划生育科。

参与者

207名临床疑似宫颈癌（CxCa）或确诊CxCa/癌前病变病例的转诊患者。

方法

从207份检测24种HPV基因型的样本中，收集（2018年10月至2020年11月）HPV - 18（40份样本）和/或HPV - 45（28份样本）L1 DNA呈阳性个体的宫颈拭子。从便利样本中提取DNA，并对HPV - E6/E7进行PCR（33/40 - HPV - 18阳性或20/28 - HPV - 45阳性样本）、测序及BLAST分析。

结果

PCR扩增后，33份（HPV - 18阳性）样本中的26份和20份（HPV - 45阳性）样本中的部分样本的E6/E7基因区域符合测序要求。对于HPV - 18变体，26份样本中的24份（92.31%）属于B/C谱系，包括C谱系样本和22份B谱系样本（其中部分样本存在E7 - SNP - C665T）。10份HPV - 45变体中有9份属于A1亚谱系，其中部分样本同时存在E6 - SNPs - C134T和C4I7T，包括1份同时检测到E6 - SNPs - C134T、G415C、C4I7T的样本。

结论

我们的研究证实加纳HPV - 45 - A1亚谱系和HPV - 18 - B谱系（非洲特异性HPV - 18 - C谱系罕见）变体占主导地位。我们的研究提供了加纳CxCa病例中HPV - 18和HPV - 45谱系及亚谱系E6/E7 SNPs的初步数据。我们希望我们的数据能为未来关于HPV型特异性核苷酸变化模式和分布的研究提供信息，这可能有助于治疗干预。