Karataşlioğlu Emrah, Tosun Samet
Department of Endodontics, Faculty of Dentistry, Katip Çelebi University, İzmir, Turkey.
Department of Endodontics, Faculty of Dentistry, Pamukkale University, Denizli, Turkey.
Microsc Res Tech. 2025 Jun 30. doi: 10.1002/jemt.70007.
This study evaluated the effect of different activation methods (no activation, sonic, ultrasonic, and XP-Endo Finisher) on the penetration depth and area of AH Plus and MTA Fillapex sealers. Two hundred single-rooted teeth were prepared and randomly divided into eight groups (n = 25). Sealers stained with rhodamine B were inserted and activated as follows: Group 1 (control, no activation, G1A, G1M), Group 2 (sonic: G2A, G2M), Group 3 (ultrasonic: G3A, G3M), and Group 4 (XP-Endo Finisher: G4A, G4M). The single cone technique was used for filling, and specimens were sectioned perpendicularly. Confocal laser scanning microscopy (CLSM) measured penetration depth, while penetration area was calculated as the sealer's penetration relative to the canal area. Two-way ANOVA was used for statistical analysis. Ultrasonic and XP-Endo Finisher activation significantly increased the penetration depth of MTA Fillapex compared to the control group (p < 0.05), while no significant difference was found for AH Plus (p > 0.05). MTA Fillapex also demonstrated a significantly greater penetration area than AH Plus when ultrasonic and XP-Endo Finisher activation were applied (p < 0.05). No significant differences were observed between sealers in the control and sonic groups (p > 0.05). Activation methods did not significantly affect the penetration area of AH Plus (p > 0.05). XP-Endo Finisher and ultrasonic activation significantly improved both penetration depth and area for MTA Fillapex but had limited effect on AH Plus. These findings highlight the clinical importance of selecting the appropriate activation technique based on the type of sealer used, as enhanced penetration may contribute to improved sealing ability and long-term endodontic success.
本研究评估了不同激活方法（未激活、声波激活、超声激活和XP-Endo Finisher）对AH Plus和MTA Fillapex封闭剂的渗透深度和面积的影响。制备了200颗单根牙，并随机分为八组（n = 25）。将用罗丹明B染色的封闭剂插入并按以下方式激活：第1组（对照组，未激活，G1A，G1M），第2组（声波激活：G2A，G2M），第3组（超声激活：G3A，G3M），第4组（XP-Endo Finisher激活：G4A，G4M）。采用单锥技术进行充填，并将标本垂直切片。共聚焦激光扫描显微镜（CLSM）测量渗透深度，而渗透面积计算为封闭剂相对于根管面积的渗透量。采用双向方差分析进行统计分析。与对照组相比，超声激活和XP-Endo Finisher激活显著增加了MTA Fillapex的渗透深度（p < 0.05），而AH Plus未发现显著差异（p > 0.05）。当采用超声激活和XP-Endo Finisher激活时，MTA Fillapex的渗透面积也显著大于AH Plus（p < 0.05）。对照组和声波激活组的封闭剂之间未观察到显著差异（p > 0.05）。激活方法对AH Plus的渗透面积没有显著影响（p > 0.05）。XP-Endo Finisher和超声激活显著提高了MTA Fillapex的渗透深度和面积，但对AH Plus的影响有限。这些发现凸显了根据所用封闭剂类型选择合适激活技术的临床重要性，因为增强的渗透可能有助于提高封闭能力和根管治疗的长期成功率。
