Immunological and inflammatory responses in the kidneys in experimental acanthamoebiasis.

The pathomechanisms of spp. infection are still poorly understood. The aim of the study was to determine the expression of the NLR family pyrin domain-containing protein 3 (NLRP3), prostaglandin-endoperoxide synthase 2 (PTGS2, commonly known as cyclooxygenase-2, COX-2), and various cytokines in the kidneys of immunocompetent and immunosuppressed mice infected with sp. (T16 genotype). The proteins were analyzed by quantitative reverse transcription PCR. In immunocompetent mice, we observed increased mRNA levels of interferon gamma (IFNγ), tumor necrosis factor alpha (TNFα), PTGS2/COX-2, and interleukin (IL)-17A at the beginning of infection. While in the late stages, we found decreased levels of NLRP3, IL-1β, IL-10, IL-17A, IL-21, IL-23, IFNγ, TNFα, and macrophage inflammatory protein-2 (MIP-2) in the kidneys of infected hosts compared to uninfected ones. In immunosuppressed mice, we noted higher expressions of NLRP3, PTGS2/COX-2, IL-1β, IL-10, IL-17A, IL-21, IFNγ, TNFα, and MIP-2, and lower expression of IL-18 in the infected mice compared to the control group. The basic understanding of the immune response during sp. infection is critical to improving clinical outcomes and also has significant implications for developing therapeutic interventions. Here, we have reported the inflammatory responses in the kidneys infected with sp. However, additional studies are needed to understand the specific beneficial and detrimental roles of the cytokine response.IMPORTANCE spp. are significant biological factors and can cause rare infections characterized by high mortality and difficulty with treatment. One of the reasons spp. persist so effectively in the body is a lack of knowledge about the pathomechanisms and pathophysiology of infections. Recent studies showed that spp. can also infect the kidneys in the hosts, being an important cause of medical complications. A better understanding of the mechanisms by which spp. cause kidney injury could lead to more effective treatments for systemic acanthamoebiasis.