Ribeiro Ana Elisa, Monteiro Naice Eleidiane Santana, Costa Paiva Lucia Helena Simões da, Pedro Adriana Orcesi
Department of Obstetrics and Gynecology, State University of Campinas (UNICAMP- Universidade Estadual de Campinas), Campinas, São Paulo, Brazil.
Food & Nutrition Department, School of Food Engineering, University of Campinas (UNICAMP-Universidade Estadual de Campinas), Campinas, São Paulo, Brazil.
Menopause. 2025 Jul 1;32(7):610-620. doi: 10.1097/GME.0000000000002544.
To correlate the urinary excretion of equol with intestinal microbiota in response to isoflavone supplementation alone or in combination with probiotics, and to compare these results with those of hormone therapy in postmenopausal women.
A pilot study was conducted on 47 postmenopausal women aged 40-60 years. Participants were randomly assigned to one of three groups: (A) oral isoflavone (150 mg dry extract of Glycine max); (B) isoflavone plus probiotic (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, Bifidobacterium bifidum, and Bifidobacterium lactis); or (C) hormone therapy (1 mg estradiol/0.5 mg norethisterone acetate). Fecal samples were collected to extract bacterial DNA, and equol and its intermediates were measured using gas chromatography coupled with mass spectrometry at baseline and after 16 weeks of treatment.
In the isoflavone alone and isoflavone plus probiotic groups, no statistically significant correlations were found between bacterial phyla or genera and the urinary excretion of equol or its intermediate after 16 weeks of treatment. In the hormone therapy group, directly proportional correlation between Bacteroidetes (Spearman R=0.83, Pa=0.01), Cyanobacteria (Spearman R=0.89, Pa=0.002), Alistipes (Spearman R=0.81, Pa=0.04), Catenibacterium (Spearman R=0.81, Pa=0.04), Clostridium (Spearman R=0.84, Pa=0.02), Barnesiella (Spearman R=0.84, Pa=0.02), and the urinary excretion of the equol intermediate were observed.
Only the hormone therapy group showed a significant increase in bacterial abundance involved in isoflavone metabolism.
将异黄酮单独或与益生菌联合补充后雌马酚的尿排泄量与肠道微生物群进行关联，并将这些结果与绝经后女性激素治疗的结果进行比较。
对47名年龄在40 - 60岁的绝经后女性进行了一项初步研究。参与者被随机分配到三组中的一组：(A)口服异黄酮（150毫克大豆干提取物）；(B)异黄酮加益生菌（嗜酸乳杆菌、干酪乳杆菌、乳酸乳球菌、双歧双歧杆菌和乳酸双歧杆菌）；或(C)激素治疗（1毫克雌二醇/0.5毫克醋酸炔诺酮）。采集粪便样本以提取细菌DNA，并在基线和治疗16周后使用气相色谱-质谱联用仪测量雌马酚及其中间体。
在单独使用异黄酮组和异黄酮加益生菌组中，治疗16周后，细菌门或属与雌马酚或其中间体的尿排泄量之间未发现统计学上的显著相关性。在激素治疗组中，观察到拟杆菌门（斯皮尔曼相关系数R = 0.83，P值 = 0.01）、蓝细菌（斯皮尔曼相关系数R = 0.89，P值 = 0.002）、阿利斯杆菌属（斯皮尔曼相关系数R = 0.81，P值 = 0.04）、链状杆菌属（斯皮尔曼相关系数R = 0.81，P值 = 0.04）、梭菌属（斯皮尔曼相关系数R = 0.84，P值 = 0.02）、巴恩斯氏菌属（斯皮尔曼相关系数R = 0.84，P值 = 0.02）与雌马酚中间体的尿排泄量呈正比关系。
只有激素治疗组显示出参与异黄酮代谢的细菌丰度显著增加。
