Moradi Kooshkmeydani Mahdi, Khoradmehr Arezoo, Dehghani Firoozabadi Ali, Tavassoli Alireza, Mohammad Rezazadeh Farzad, Mohebbi Gholamhossein, A Kaliyev Asset, Mussin Nadiar Maratovich, Farjam Mojtaba, R Zhilisbayeva Kulyash, Tamadon Amin
Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran.
Persian Gulf Marine Biotechnology Research Center, Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran.
Int J Fertil Steril. 2025 May 14;19(3):326-336. doi: 10.22074/ijfs.2024.2019125.1602.
Cervical, breast, and ovarian cancers exhibit significant incidence and fatality rates, necessitating diverse approaches for effective cancer cell eradication while preserving normal cells. The aim of this study is to explore the apoptosis-inducing properties of hydroalcoholic extracts from (C. rotundus) leaf using human gynecological cancer cell lines.
In this experimental study, Hydroalcoholic extracts were prepared from the leaf of C. rotundus using ethanol, methanol, and ethyl acetate. These extracts were applied to MCF-7, HeLa, OVCAR-3, and Vero cell lines at concentrations of 3.125, 6.25, 12.5, and 25 g/ml. MTT test, assessing inhibition of proliferation at 50% (IC), was employed to evaluate each extract's ability to inhibit cell proliferation. Subsequently, apoptosis-related gene and protein regulation were examined using reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis.
Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the methanolic extract contained hexadecanoic acid and dodecanoic acid. The ethanolic extract was found to have norspermidine and desulphosinigrin. Additionally, the ethyl acetate extract included vitamin E, 1-heptatriacotanol, lupeol, betulin, stigmasterol, and stearic acid. The HeLa treatment group with 6.25 μg/ml of ethyl acetate extract, MCF-7, and OVCAR-3 cells with 3.125 μg/ ml of methanol extract treatment group exhibited the most significant growth inhibition in the MTT assay. Further analysis of these treatment groups revealed that the transcription and translation of BAX, Caspase-3, Caspase-8, and Caspase-9 increased overall, whereas Bcl-2 decreased in all cell lines.
Hydroalcoholic extracts from leaf may enhance the apoptosis of cancer cells by modulating the transcription, translation, and post-translation of proteins, with minimal impact on the growth and survival of non-cancerous cells.
宫颈癌、乳腺癌和卵巢癌的发病率和死亡率都很高，因此需要采用多种方法在保留正常细胞的同时有效根除癌细胞。本研究的目的是利用人妇科癌细胞系探索圆叶锦葵叶水醇提取物的诱导凋亡特性。
在本实验研究中，使用乙醇、甲醇和乙酸乙酯从圆叶锦葵叶中制备水醇提取物。这些提取物以3.125、6.25、12.5和25 g/ml的浓度应用于MCF-7、HeLa、OVCAR-3和Vero细胞系。采用MTT试验评估50%抑制浓度（IC）下的增殖抑制情况，以评估每种提取物抑制细胞增殖的能力。随后，使用逆转录聚合酶链反应（RT-PCR）和蛋白质印迹分析检测凋亡相关基因和蛋白质的调控情况。
气相色谱-质谱（GC-MS）分析表明，甲醇提取物含有十六烷酸和十二烷酸。乙醇提取物含有亚精胺和脱硫黑芥子苷。此外，乙酸乙酯提取物包括维生素E、1-二十七醇、羽扇豆醇、桦木醇、豆甾醇和硬脂酸。在MTT试验中，用6.25 μg/ml乙酸乙酯提取物处理的HeLa组、用3.125 μg/ml甲醇提取物处理的MCF-7组和OVCAR-3组表现出最显著的生长抑制。对这些处理组的进一步分析表明，所有细胞系中BAX、Caspase-3、Caspase-8和Caspase-9的转录和翻译总体上增加，而Bcl-2减少。
圆叶锦葵叶水醇提取物可能通过调节蛋白质的转录、翻译和翻译后过程来增强癌细胞的凋亡，对非癌细胞的生长和存活影响最小。
