Zhang Huanhuan, Li Linmei, Yang Siqi, Xue Bowei, Yan Shangbo, Hu Chun, Li Qing, Shen Weishou, Gao Nan
School of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, and School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing 210044, China.
J Appl Microbiol. 2025 Jul 1;136(7). doi: 10.1093/jambio/lxaf162.
Acidic soils have a high potential for nitrous oxide (N2O) emission. Plant growth-promoting rhizobacteria (PGPR) may mitigate N2O emissions in acidic soils; however, the reduction capacity and their microbiological mechanisms are poorly understood. We investigated the effects of three PGPR strains (Bacillus subtilis subsp. subtilis NRCB002, Stutzerimonas stutzeri NRCB010, and B. velezensis NRCB026) on crop growth and N2O emissions in acidic soils, and explored their microbial mechanisms.
A field experiment was conducted to assess the effects of inoculation with NRCB002, NRCB010, and NRCB026 on lettuce growth and N2O emissions from acidic soil and to study the associated microbiological mechanisms. The results indicated that inoculation with NRCB002, NRCB010, and NRCB026 significantly promoted lettuce growth and decreased N2O emissions from soil by 16.3%, 36.2%, and 45.8%, respectively. Furthermore, inoculation with these three strains altered the microbial community composition and nitrogen cycle functional gene abundance in acidic soils. Structural equation models indicated that inoculation with these three strains affected N2O emissions from the soil by modulating soil pH, relative abundance of Proteobacteria, and nosZ gene copy numbers. N2O emissions from soil inoculated with NRCB002 were negatively correlated with nosZI gene copy number, while NRCB010 and NRCB026 were negatively correlated with nosZII gene copy number.
PGPR strains enhanced lettuce growth and decreased acidic soil N2O emissions under field conditions. Among the three strains, NRCB026 significantly decreased N2O emission from acidic soil.
酸性土壤具有较高的一氧化二氮（N2O）排放潜力。植物促生根际细菌（PGPR）可能会减少酸性土壤中的N2O排放；然而，其减排能力及其微生物学机制尚不清楚。我们研究了三种PGPR菌株（枯草芽孢杆菌枯草亚种NRCB002、施氏假单胞菌NRCB010和贝莱斯芽孢杆菌NRCB026）对酸性土壤中作物生长和N2O排放的影响，并探讨了其微生物学机制。
进行了一项田间试验，以评估接种NRCB002、NRCB010和NRCB026对生菜生长以及酸性土壤N2O排放的影响，并研究相关的微生物学机制。结果表明，接种NRCB002、NRCB010和NRCB026显著促进了生菜生长，并使土壤N2O排放量分别降低了16.3%、36.2%和45.8%。此外，接种这三种菌株改变了酸性土壤中的微生物群落组成和氮循环功能基因丰度。结构方程模型表明，接种这三种菌株通过调节土壤pH值、变形菌门的相对丰度和nosZ基因拷贝数来影响土壤中的N2O排放。接种NRCB002的土壤中N2O排放与nosZI基因拷贝数呈负相关，而NRCB010和NRCB026与nosZII基因拷贝数呈负相关。
在田间条件下，PGPR菌株促进了生菜生长并降低了酸性土壤的N2O排放。在这三种菌株中，NRCB026显著降低了酸性土壤中的N2O排放。
