Attias Marcia, de Souza Wanderley, Dubey Jitender P
Laboratório de Ultraestrutura Celular Hertha Meyer, Centro de Pesquisa em Medicina de Precisão, Instituto de Biofísica Carlos Chagas Filho. Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Centro Nacional de Biologia Estrutural e Bioimagem-CENABIO. Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Cell Tissue Res. 2025 Sep;401(3):287-295. doi: 10.1007/s00441-025-03988-w. Epub 2025 Jul 1.
This study investigates the enteroepithelial stages of Toxoplasma gondii in the feline ileum using high resolution field emission scanning electron microscopy (FE-SEM) and serial block face scanning electron microscopy (SBF-SEM). By employing advanced imaging techniques, including creative sample processing methods, this research provides new insights into the development, distribution, and maturation of T. gondii within infected intestinal cells. Comparison with previous studies confirms earlier findings while offering enhanced resolution and three-dimensional models of the parasite's progression. SBF-SEM enabled the visualization of asexual forms of the parasite, from merozoites to multinucleated schizonts and rosettes, distributed along the intestinal villi. These observations suggest a tendency for merozoites to accumulate near the villus extrusion zone, where they are later released. Despite these advancements, the mechanisms of gamete fertilization and oocyst egress remain unresolved, highlighting key areas for future research.
本研究使用高分辨率场发射扫描电子显微镜（FE-SEM）和连续块面扫描电子显微镜（SBF-SEM），对猫回肠中刚地弓形虫的肠上皮阶段进行了研究。通过采用先进的成像技术，包括创新性的样本处理方法，本研究为刚地弓形虫在受感染肠细胞内的发育、分布和成熟提供了新的见解。与先前研究的比较证实了早期的发现，同时提供了更高分辨率以及寄生虫发育过程的三维模型。SBF-SEM能够可视化寄生虫的无性形态，从裂殖子到多核裂殖体和玫瑰花结，它们沿着肠绒毛分布。这些观察结果表明，裂殖子有在绒毛挤出区附近聚集的趋势，随后它们从那里释放。尽管取得了这些进展，但配子受精和卵囊排出的机制仍未得到解决，这突出了未来研究的关键领域。
