Suppr超能文献

补充铜可增强色素沉着并诱导ARPE19细胞产生多巴胺。

Copper supplementation enhances pigmentation and induces dopamine production in ARPE19.

作者信息

Uehara Hironori, Shakaib Baila, Kumar Sangeetha Ravi, Archer Bonnie, Ambati Balamurali

机构信息

Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon, Eugene, Oregon, United States of America.

出版信息

PLoS One. 2025 Jul 1;20(7):e0327352. doi: 10.1371/journal.pone.0327352. eCollection 2025.

Abstract

Non-neuronal dopamine production has not been understood despite dopamine function in non-neuronal tissues. Tyrosinase is a non-neuronal enzyme which converts tyrosine to L-DOPA (l-3,4-dihydroxyphenylalanine) and L-DOPA to l-dopaquinone for further melanin production. Since L-DOPA is a dopamine precursor in neurons, we hypothesized that tyrosinase-derived L-DOPA could alternatively be converted to dopamine. Therefore, this study investigated whether copper supplementation enhanced pigmentation and induced dopamine production via tyrosinase activation in APRE19 cells. Copper is known as a tyrosinase cofactor. In two separate experiments, we cultured ARPE19 in 1% FBS/DMEM with/without 10 μM copper sulfate for approximately 100 days. After 40-50 days, slight pigmentation with copper treatment was confirmed in the cell pellets, while no pigmentation was observed in the non-copper control. After 90-100 days, the pigmentation in the copper treatment group was obvious, while minimal pigmentation was observed in the non-copper control. Dopamine was not detected at 40-50 days in either group, while it was detected after 90-100 days of culture only in the copper-treated group. Tyrosinase mRNA expression was confirmed in both groups at a similar level, while tyrosinase protein expression was significantly higher in the copper treatment group than in the non-copper control. Thus, we determined that copper supplementation efficiently enhances pigmentation and induces dopamine production in long-term culture ARPE19, likely due to increased tyrosinase protein expression and activity. This is the first report showing the significance of copper in non-neuronal dopamine production of RPE cells, which suggests that tyrosinase may be responsible for non-neuronal dopamine production.

摘要

尽管多巴胺在非神经元组织中发挥作用,但非神经元多巴胺的产生机制仍未明确。酪氨酸酶是一种非神经元酶,可将酪氨酸转化为L-多巴(l-3,4-二羟基苯丙氨酸),并将L-多巴转化为l-多巴醌以进一步合成黑色素。由于L-多巴是神经元中多巴胺的前体,我们推测酪氨酸酶衍生的L-多巴可能会转化为多巴胺。因此,本研究探讨了补充铜是否能通过激活APRE19细胞中的酪氨酸酶来增强色素沉着并诱导多巴胺生成。铜是已知的酪氨酸酶辅因子。在两个独立实验中,我们将ARPE19细胞培养于含1%胎牛血清的杜氏改良 Eagle 培养基(DMEM)中,分别添加或不添加10 μM硫酸铜,培养约100天。40 - 50天后,在细胞沉淀中证实铜处理组有轻微色素沉着,而未加铜的对照组未观察到色素沉着。90 - 100天后,铜处理组色素沉着明显,而未加铜的对照组色素沉着极少。两组在40 - 50天时均未检测到多巴胺,而仅在培养90 - 100天后,铜处理组检测到多巴胺。两组酪氨酸酶mRNA表达水平相似,而铜处理组酪氨酸酶蛋白表达显著高于未加铜的对照组。因此,我们确定在长期培养的ARPE19细胞中,补充铜能有效增强色素沉着并诱导多巴胺生成,这可能是由于酪氨酸酶蛋白表达和活性增加所致。这是首次报道铜在视网膜色素上皮(RPE)细胞非神经元多巴胺生成中的重要作用,提示酪氨酸酶可能参与非神经元多巴胺的生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4cc1/12212581/d25d31c12735/pone.0327352.g001.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验