双氢青蒿素通过抑制RalB表达抑制舌鳞状细胞癌进展及舌至淋巴结转移。
Dihydroartemisinin inhibited tongue squamous cell carcinoma progression and tongue-to-lymph node metastasis through inhibiting RalB expression.
作者信息
Zhang Yuman, Gao Yuting, Gong Yanli, Yang Yanguang, Gong Yi, Song Xiaoyong, Xiong Yajun, Wang Dan, Liu Zhihan, Shi Xinli
机构信息
Department of Pathobiology and Immunology, Hebei University of Chinese Medicine, Shijiazhuang 050200, China.
Department of Pathobiology and Immunology, Hebei University of Chinese Medicine, Shijiazhuang 050200, China; Laboratory of Integrated Medicine Tumor Immunology, Shanxi University of Chinese Medicine, Taiyuan 030000, China.
出版信息
Acta Histochem. 2025 Sep;127(3):152276. doi: 10.1016/j.acthis.2025.152276. Epub 2025 Jun 30.
BACKGROUND
Lymph node metastasis is a key determinant of the poor survival rate in patients with tongue squamous cell carcinoma (TSCC). Therefore, inhibiting lymph node metastasis is a primary strategy for TSCC treatment. Our previous research found that dihydroartemisinin (DHA) inhibited the migration in human tongue squamous carcinoma Cal-27 cells. However, the effect and mechanism of DHA on lymph node metastasis are unknown in TSCC.
METHODS
The expression level of Ras related GTP binding protein B (RalB) was measured in TSCC samples by immunohistochemical staining. Wound healing, invasion, and cell adhesion assays were used to investigate cell motility. Western blot was used to investigate RalB expression level. An orthotopic nude mouse model of TSCC was established to investigate the effect and mechanism of DHA on lymph node metastasis.
RESULTS
First, DHA inhibited the progression of tongue tumor and tongue-to-lymph node metastasis of TSCC. Secondly, DHA inhibited RalB expression level in vitro and in vivo. Finally, DHA inhibited tongue-to-lymph node metastasis through RALB.
CONCLUSIONS
DHA inhibited tongue-to-lymph node metastasis through RALB, providing a novel therapeutic strategy for TSCC metastasis.
背景
淋巴结转移是影响舌鳞状细胞癌（TSCC）患者生存率的关键因素。因此，抑制淋巴结转移是TSCC治疗的主要策略。我们之前的研究发现双氢青蒿素（DHA）可抑制人舌鳞状癌细胞Cal-27的迁移。然而，DHA对TSCC淋巴结转移的影响及其机制尚不清楚。
方法
采用免疫组化染色法检测TSCC样本中Ras相关GTP结合蛋白B（RalB）的表达水平。采用伤口愈合、侵袭和细胞黏附实验研究细胞运动能力。采用蛋白质免疫印迹法检测RalB的表达水平。建立TSCC原位裸鼠模型，研究DHA对淋巴结转移的影响及其机制。
结果
首先，DHA抑制了TSCC的舌肿瘤进展及舌至淋巴结转移。其次，DHA在体外和体内均抑制了RalB的表达水平。最后，DHA通过RALB抑制舌至淋巴结转移。
结论
DHA通过RALB抑制舌至淋巴结转移，为TSCC转移提供了一种新的治疗策略。
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