Jung Doo Yeon, Lee Hyun Jung, Lee Young-Seung, Kim Minsu, Lee Chang-Kyu, Jo Cheorun
Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University, Seoul, Republic of Korea.
Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang, Republic of Korea.
NPJ Sci Food. 2025 Jul 1;9(1):115. doi: 10.1038/s41538-025-00480-1.
Optimizing the metabolic environment during porcine skeletal muscle stem cells (PSCs) proliferation and differentiation is essential for efficient muscle cell cultivation. This study investigated metabolic shifts during myogenic progression using cells at P1, P3, D1, and D2 for characterization and metabolomic analysis. Cell number increased until P3, and myotubes formed by D2 but detached by D3 in 2D culture. Gene and protein expression analyses confirmed myogenic progression, including increased MYOD and MHC expression. Metabolomic profiling identified 94 intracellular metabolites, with 24 and 17 differentially abundant metabolites (DAMs) in proliferation and differentiation, respectively. Pathway analysis showed that energy production via amino acid metabolism was key to progression. Notably, PSCs relied on glycolysis during proliferation and shifted to mitochondrial oxidative phosphorylation during differentiation. These results highlight stage-specific metabolic reprogramming and provide insights for optimizing culture conditions, offering foundational knowledge to improve the efficiency and quality of cultured meat production.
在猪骨骼肌干细胞（PSCs）增殖和分化过程中优化代谢环境对于高效培养肌肉细胞至关重要。本研究使用P1、P3、D1和D2阶段的细胞进行表征和代谢组学分析，以研究成肌过程中的代谢变化。在二维培养中，细胞数量增加至P3，D2时形成肌管，但D3时肌管脱离。基因和蛋白质表达分析证实了成肌过程，包括MYOD和MHC表达增加。代谢组学分析鉴定出94种细胞内代谢物，增殖和分化过程中分别有24种和17种差异丰富代谢物（DAMs）。通路分析表明，通过氨基酸代谢产生能量是成肌过程的关键。值得注意的是，PSCs在增殖过程中依赖糖酵解，在分化过程中转变为线粒体氧化磷酸化。这些结果突出了阶段特异性代谢重编程，并为优化培养条件提供了见解，为提高 cultured meat生产的效率和质量提供了基础知识。
