使用表面等离子体共振的结合动力学和表位分组
Binding Kinetics and Epitope Binning Using Surface Plasmon Resonance.
作者信息
Nilvebrant Johan
机构信息
Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.
出版信息
Methods Mol Biol. 2025;2937:277-295. doi: 10.1007/978-1-0716-4591-8_17.
PMID:40593427
Abstract
The ability to quantify binding affinity of molecular interactions is an essential component of drug development and life science research. This chapter outlines the practical use of surface plasmon resonance spectroscopy to monitor protein-protein interactions with an emphasis on basic experimental design. A short overview of common epitope binning assays is also included.
摘要
量化分子相互作用结合亲和力的能力是药物开发和生命科学研究的重要组成部分。本章概述了表面等离子体共振光谱在监测蛋白质-蛋白质相互作用中的实际应用，重点介绍了基本实验设计。还包括对常见表位分组分析的简要概述。
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