Bedinger Daniel, Parisot Judicael, Ditto Noah T
Carterra Inc, Salt Lake City, UT, USA.
Methods Mol Biol. 2025;2937:325-360. doi: 10.1007/978-1-0716-4591-8_19.
Competitive epitope binning using high-throughput surface plasmon resonance (HT-SPR) is a method for understanding competitive epitope profiles of up to hundreds of monoclonal antibodies. Detailed here is an approach along with considerations for the design and analysis of a classical competitive epitope binning assay using the Carterra LSA. Monoclonal antibodies are arrayed on a biosensor chip and interrogated serially for their ability to form a trimolecular complex with antigen and solution phase monoclonal antibodies. Software tools allow the clustering of monoclonal antibodies sharing common epitopes, and the real-time nature of the data allows for study of binding profiles for each monoclonal antibody.
使用高通量表面等离子体共振（HT-SPR）进行竞争性表位分组是一种用于了解多达数百种单克隆抗体的竞争性表位谱的方法。本文详细介绍了一种使用Carterra LSA进行经典竞争性表位分组分析的设计和分析方法及注意事项。单克隆抗体被排列在生物传感器芯片上，并依次检测它们与抗原和液相单克隆抗体形成三分子复合物的能力。软件工具可对共享共同表位的单克隆抗体进行聚类，数据的实时性允许研究每种单克隆抗体的结合谱。
