通过序列设计调控DNA折纸纳米阵列的转变。
Modulating transformation of DNA origami nanoarray via sequence design.
作者信息
Wang Dongfang, Cole Fiona, Pfeiffer Martina, Cao Mengting, Schröder Tim, Tinnefeld Philip, Ke Yonggang
机构信息
School of Biomedical Engineering, University of Science and Technology of China, Hefei, China.
Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou, China.
出版信息
Nat Commun. 2025 Jul 1;16(1):5626. doi: 10.1038/s41467-025-61421-w.
The four-way DNA junction is the most prevalent structural motif in DNA nanotechnology. Recently, a reconfigurable DNA nanoarray (domino array) was created with this basic motif to realize intricate, stepwise transformation by the information relay between neighboring four-way junction units. Here, we generate a DNA domino array with same sequences at every junction, and use it as a platform to study how the design of DNA bases at junctions influences the kinetics and thermodynamics of transformation of four-way junctions in reconfigurable DNA nanoarrays. By regulating the energy difference and thus the conversion between the two configurations of four-way junctions, we show the transformation of DNA nanoarray can be modulated in a designable manner. The coordinated transformation of four-way junctions in the DNA domino array enables a detailed investigation on array transformation by using Atomic Force Microscopy (AFM) imaging and single-molecule Förster resonance energy transfer (FRET) microscopy.
四向DNA连接体是DNA纳米技术中最普遍的结构基序。最近，利用这一基本基序构建了一种可重构DNA纳米阵列（多米诺阵列），通过相邻四向连接体单元之间的信息传递实现复杂的逐步转化。在此，我们构建了一种在每个连接点具有相同序列的DNA多米诺阵列，并将其作为一个平台，来研究连接点处DNA碱基的设计如何影响可重构DNA纳米阵列中四向连接体转化的动力学和热力学。通过调节能量差以及四向连接体两种构型之间的转换，我们表明DNA纳米阵列的转化可以以一种可设计的方式进行调节。DNA多米诺阵列中四向连接体的协同转化使得利用原子力显微镜（AFM）成像和单分子荧光共振能量转移（FRET）显微镜对阵列转化进行详细研究成为可能。
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