Alimullah Mirza, Jahan Ishrat, Rahman Md Mizanur, Khan Ferdous, Shahin Ahmed Khondoker, Hossain Md Hemayet, Arabnozari Hesamoddin, Alam Md Ashraful, Sarker Satyajit D, Nahar Lutfun, Subhan Nusrat
Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh.
BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka, Bangladesh.
Sci Rep. 2025 Jul 1;15(1):20859. doi: 10.1038/s41598-025-08720-w.
Myocardial infarction is a leading cause of death and morbidity in individuals with cardiovascular diseases. Natural antioxidants, such as those found in green tea leaves, are beneficial in preventing these diseases. This study evaluated the protective effects of green tea leaves powder against isoprenaline (ISO)-induced myocardial infarction in rats. Four groups of male Long Evans rats were used: Control, Control + green tea leaves powder, ISO, and ISO + green tea leaves powder. Organ and blood plasma samples were collected to measure oxidative stress biomarkers, biochemical parameters, and gene expressions. Furthermore, tissue sections were prepared and stained histologically. ISO-induced rats showed decreased cellular antioxidants (catalase activity and glutathione concentration) and elevated oxidative stress markers. Notable inflammatory cell infiltration and fibrosis were observed in the heart and kidneys of ISO-induced rats. Supplementation with green tea leaves powder significantly restored catalase activity and glutathione concentration (p < 0.05) in plasma and tissues. It also considerably reduced lipid peroxidation, nitric oxide, and advanced oxidation protein products (p < 0.05) in ISO-administered rats. Furthermore, green tea leaves powder supplementation halted inflammatory gene expression (p < 0.05), restored antioxidant genes (p < 0.05) such as Nrf-2-HO-1, and prevented cardiac fibrosis in ISO-administered rats. Green tea leaves powder supplementation may reduce oxidative stress, inflammation, and fibrosis in ISO-administered rats, potentially through the Nrf-2-HO-1-mediated restoration of antioxidant enzymes and prevention of heart inflammation.
心肌梗死是心血管疾病患者死亡和发病的主要原因。天然抗氧化剂，如绿茶中所含的抗氧化剂，有助于预防这些疾病。本研究评估了绿茶粉对异丙肾上腺素（ISO）诱导的大鼠心肌梗死的保护作用。使用了四组雄性朗·埃文斯大鼠：对照组、对照组 + 绿茶粉组、ISO组和ISO + 绿茶粉组。收集器官和血浆样本以测量氧化应激生物标志物、生化参数和基因表达。此外，制备组织切片并进行组织学染色。ISO诱导的大鼠表现出细胞抗氧化剂（过氧化氢酶活性和谷胱甘肽浓度）降低以及氧化应激标志物升高。在ISO诱导的大鼠的心脏和肾脏中观察到明显的炎症细胞浸润和纤维化。补充绿茶粉可显著恢复血浆和组织中的过氧化氢酶活性和谷胱甘肽浓度（p < 0.05）。它还显著降低了ISO给药大鼠的脂质过氧化、一氧化氮和晚期氧化蛋白产物（p < 0.05）。此外，补充绿茶粉可阻止炎症基因表达（p < 0.05），恢复抗氧化基因（p < 0.05），如Nrf-2-HO-1，并预防ISO给药大鼠的心脏纤维化。补充绿茶粉可能通过Nrf-2-HO-1介导的抗氧化酶恢复和心脏炎症预防作用，减轻ISO给药大鼠的氧化应激、炎症和纤维化。
