Itoh S, Yamaba Y, Matsuo S, Oda T, Satoh T
Res Commun Chem Pathol Pharmacol. 1985 Sep;49(3):447-50.
To study the relationship between the activity of nonspecific carboxylesterase located in the smooth endoplasmic reticulum (SER) of hepatocytes, and serum thyroid hormones including triiodothyronine (T3), thyroxine (T4), and T3/T4 ratio, we determined these hormones, liver carboxylesterase activity, and serum alanine amino-transferase (SGPT) before, and 5 to 720 min after treatment with a single oral dose of CC14 (1 ml/kg) in rats. The serum T3/T4 ratio decreased 5 to 10 min after administration. Each of 16 male and 16 female rats showed a significant correlation between carboxylesterase activity and T3/T4 ratio (male, r = 0.79, p less than 0.01; female, r = 0.84, p less than 0.01), but revealed no correlation between carboxylesterase and T3 or T4, alone. SGPT contents were elevated 6 to 12 h after, whereas T3/T4 ratio decreased gradually during the course. The SER increased mildly 5 min after, but was irregularly distended 6 h after. Changes in the microvilli were not found 5 min after, but the disruption was seen 6 h after. These results suggest that serum T3/T4 ratio is closely correlated with microsomal carboxylesterase activity in CC14-treated rats and represents a microsomal function.
为研究位于肝细胞滑面内质网（SER）的非特异性羧酸酯酶活性与血清甲状腺激素（包括三碘甲状腺原氨酸（T3）、甲状腺素（T4）及T3/T4比值）之间的关系，我们测定了大鼠单次口服给予四氯化碳（CC14，1 ml/kg）前及给药后5至720分钟的这些激素、肝脏羧酸酯酶活性及血清谷丙转氨酶（SGPT）。给药后5至10分钟血清T3/T4比值降低。16只雄性大鼠和16只雌性大鼠的羧酸酯酶活性与T3/T4比值均呈显著相关（雄性，r = 0.79，p < 0.01；雌性，r = 0.84，p < 0.01），但羧酸酯酶与单独的T3或T4无相关性。SGPT含量在给药后6至12小时升高，而T3/T4比值在此过程中逐渐降低。给药后5分钟SER轻度增加，但6小时后不规则扩张。给药后5分钟未发现微绒毛变化，但6小时后可见微绒毛破坏。这些结果表明，在CC14处理的大鼠中，血清T3/T4比值与微粒体羧酸酯酶活性密切相关，代表一种微粒体功能。
