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拟南芥中TALE（三氨基酸环扩展）转录因子的全基因组鉴定、表达模式及功能分析

Genome-wide identification, expression patterns and functional analysis of TALE (Three Amino-Acid Loop Extension) transcription factors in Arabidopsis thaliana.

作者信息

Ali Hina, Liu Yanhui, Azam Syed Muhammad, Ahmad Mayra, Pei Mengtian, Olsson Stefan, Ullah Riaz, Shahat Abdelaaty A

机构信息

Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China.

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

出版信息

BMC Plant Biol. 2025 Jul 2;25(1):796. doi: 10.1186/s12870-024-05861-4.

DOI:10.1186/s12870-024-05861-4

PMID:40596813

Abstract

BACKGROUND

Members of the TALE gene family play a crucial part as transcription factors, governing flower organs development, organ morphogenesis, flower meristem organization, and the development of fruits. However, detailed genome-wide study and functional analysis of TALE transcription factors has not yet been conducted in the model plant A. thaliana.

RESULTS

A total of 21 genes belonging to the A. thaliana TALE gene family was identified, separated into two subfamilies (BELL and KNOX), and analyzed using bioinformatics methods, providing a theoretical basis for functional research and application of A. thaliana TALE genes. TALE genes were found on four chromosomes in A. thaliana. The A. thaliana TALE genes exhibited structural heterogeneity. All AtTALEs possessed a BELL and a KNOX domain, along with conservative structures. The A. thaliana TALE gene family had several cis-elements in response to abiotic stress and hormones, showing that the majority of AtTALE genes were involved in A. thaliana growth and development as well as stress. The expression of TALE genes in A. thaliana was shown to be tissue-specific. RNA-seq data revealed that some TALE genes were highly expressed in response to abiotic stresses. Furthermore, a functional study of the TALE gene (knat3) showed a significant reduction in silique length, seed set and weak plant stature. We found defects in female gametophyte developmental stages, suggesting the possible role of the TALE genes in female gametophyte development. While in vitro studies of knat3 mutant pollen germination showed non-viable pollen grains (30%), indicating a putative involvement of TALE genes in male gametophyte development.

CONCLUSION

This study investigated TALE genes in A. thaliana and identified their potential role in response to abiotic stresses. The functional analysis of TALE gene (knat3) revealed its significant role in gametophyte development of A. thaliana, further studies are needed to confirm the TALE gene possible function in male and female gametophyte development.

摘要

背景

TALE基因家族成员作为转录因子发挥着关键作用，调控花器官发育、器官形态发生、花分生组织组织以及果实发育。然而，尚未在模式植物拟南芥中对TALE转录因子进行详细的全基因组研究和功能分析。

结果

共鉴定出21个属于拟南芥TALE基因家族的基因，分为两个亚家族（BELL和KNOX），并采用生物信息学方法进行分析，为拟南芥TALE基因的功能研究和应用提供了理论基础。拟南芥的TALE基因分布在四条染色体上。拟南芥TALE基因表现出结构异质性。所有AtTALEs都具有一个BELL结构域和一个KNOX结构域，以及保守结构。拟南芥TALE基因家族有几个响应非生物胁迫和激素的顺式元件，表明大多数AtTALE基因参与拟南芥的生长发育以及胁迫响应。TALE基因在拟南芥中的表达具有组织特异性。RNA测序数据显示，一些TALE基因在响应非生物胁迫时高表达。此外，对TALE基因（knat3）的功能研究表明，角果长度、结实率显著降低，植株矮小。我们发现雌配子体发育阶段存在缺陷，表明TALE基因可能在雌配子体发育中发挥作用。而对knat3突变体花粉萌发的体外研究表明，有30%的花粉粒无活力，表明TALE基因可能参与雄配子体发育。