Ouyang Huili, Li Xuesheng, Chen Dongyu, Zheng Xia-Lin, Lu Wen, Wang Xiaoyun
Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning, 530004, China.
Agricultural Meteorology, Guangxi Institute of Meteorological Sciences, Nanning, Guangxi, 530004, China.
BMC Genomics. 2025 Jul 1;26(1):608. doi: 10.1186/s12864-025-11800-w.
Sex differentiation is a crucial process that determines the sex ratio of a population, impacting reproductive success and ecological dynamics. In parasitoid wasps and other insects, the sex ratios significantly influence ecological adaptability and biological control potential. Particularly in parasitoid wasps, the regulation of sex ratio is closely linked to the shift between gamogenesis and parthenogenesis. However, the molecular mechanisms regulating sex ratios remain largely unresolved.
We found that when the female wasp Copidosomopsis nacoleiae (Eady) reproduces through parthenogenesis, the offspring are all male. However, when the wasp reproduces sexually, females accounted for 24.65 ± 3.64%, and males accounted for 75.35 ± 3.64%. Additionally, we identified 123,982 isoforms and predicted 5,675 alternative splicing events through transcriptomics. Interestingly, we found that the squid gene exhibited different splicing patterns in male and female offspring under different reproductive modes, suggesting that it may play a role in sex differentiation in C. nacoleiae.
Overall, the study provides important insights into the reproductive biology of C. nacoleiae and sheds light on the molecular mechanisms underlying sex differentiation in this species.
性别分化是一个关键过程，它决定了种群的性别比例，影响着繁殖成功率和生态动态。在寄生蜂和其他昆虫中，性别比例显著影响生态适应性和生物防治潜力。特别是在寄生蜂中，性别比例的调节与配子发生和孤雌生殖之间的转变密切相关。然而，调节性别比例的分子机制在很大程度上仍未得到解决。
我们发现，当雌性黄蜂纳氏寇蛛（Eady）通过孤雌生殖进行繁殖时，后代全部为雄性。然而，当黄蜂进行有性生殖时，雌性占24.65±3.64%，雄性占75.35±3.64%。此外，我们通过转录组学鉴定了123,982个异构体，并预测了5,675个可变剪接事件。有趣的是，我们发现鱿鱼基因在不同生殖模式下的雄性和雌性后代中表现出不同的剪接模式，这表明它可能在纳氏寇蛛的性别分化中发挥作用。
总体而言，该研究为纳氏寇蛛的生殖生物学提供了重要见解，并揭示了该物种性别分化的分子机制。
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