Reproductive Behavior of the Polyembryonic Parasitoid (Eady) at Different Ages.

In the control of agricultural and forestry pests, excessive reliance on chemical pesticides has led to increasingly severe issues, such as toxic residues and heightened pest resistance. The effective use of biological control has become a major focus in pest management. Parasitoid wasps, as a critical natural enemy of pests, are widely distributed, diverse in species, and play an essential role in natural pest control. , a recently discovered polyembryonic endoparasitoid wasp, parasitizes ; yet, its biological characteristics remain insufficiently studied. The artificial rearing and population propagation of this wasp have not yet been achieved, and its reproductive behavior and rhythm are not fully understood. To better utilize natural enemy resources and maximize their pest control benefits, we conducted laboratory rearing, behavioral observation, and population surveys to investigate the morphological characteristics, life history, and behavioral patterns of . Under laboratory conditions, has a generational cycle of 48.71 ± 0.48 days, with an egg-larval period of 32.17 ± 0.20 days and a pupal period of 14.36 ± 0.27 days. Adult wasps require nutritional supplementation and have a maximum lifespan of 2.18 ± 0.09 days when fed 10% honey water. The pre-mating period for adults is 4.72 ± 0.24 h, with an average mating frequency of 5.17 ± 1.65 times per lifetime. Females have a pre-oviposition period of 2.80 ± 0.31 h and an oviposition period of 4.52 ± 0.12 h, laying between 2 and 95 eggs, with an average of 12.75 ± 9.99 eggs, totaling 107.55 ± 28.38 eggs over their lifespan. Offspring production increases with the body length of the host's mature larvae. Through the successful establishment of a laboratory population of , the biological characteristics, reproductive behavior, and rhythms of were systematically examined in this study, and its occurrence dynamics in the field were investigated. These results provide a theoretical foundation for the large-scale propagation and application of to control populations effectively.