• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

单细胞测序揭示了SALL4在宫颈癌发展中的作用。

Single-cell sequencing reveals the role of SALL4 in cervical cancer development.

作者信息

Tonghui Bao, Wufen Li, Lin Qi

机构信息

Department of Gynecology, Affiliated Hospital of Qinghai University, Xining, 810000, China.

Department of Hematology and Oncology, Qinghai Provincial Traditional Chinese Medicine Hospital, Xining, 810000, China.

出版信息

BMC Cancer. 2025 Jul 1;25(1):1086. doi: 10.1186/s12885-025-14469-2.

DOI:10.1186/s12885-025-14469-2
PMID:40597906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12210988/
Abstract

OBJECTIVE

This study aims to investigate the role of SALL4 in the development and progression of cervical cancer, particularly its impact on the proliferation, migration and adhesion of HeLa cells, and to explore the clinical potential of SALL4 as a therapeutic target.

METHODS

Single-cell sequencing technology was utilized to analyze the cellular characteristics of cervical cancer tumor cell populations, and transcriptomic data were integrated to assess the differential expression of SALL4. Additionally, both in vitro and in vivo experiments were conducted to evaluate the effects of SALL4 inhibition on cell proliferation, migration, adhesion, and its regulation of collagen content and fibrosis.

RESULTS

High expression of SALL4 significantly promoted the proliferation, migration, and adhesion of cervical cancer cells. After SALL4 knockout, the migration and proliferation rates were significantly lower than those of HeLa cells. Immunofluorescence and in vivo experiments showed that SALL4 knockout cells exhibited a significantly reduced tumor formation ability, with lower proliferation and fibrosis levels compared to HeLa cells.

CONCLUSION

High expression of SALL4 promotes cervical cancer progression, while inhibition of SALL4 expression effectively suppresses cancer development. As a critical regulatory factor, SALL4 has the potential to become a therapeutic target for cervical cancer, and its application in cervical cancer treatment warrants further exploration.

摘要

目的

本研究旨在探讨SALL4在宫颈癌发生发展中的作用，特别是其对HeLa细胞增殖、迁移和黏附的影响，并探索SALL4作为治疗靶点的临床潜力。

方法

利用单细胞测序技术分析宫颈癌肿瘤细胞群体的细胞特征，并整合转录组数据评估SALL4的差异表达。此外，进行了体外和体内实验，以评估抑制SALL4对细胞增殖、迁移、黏附以及其对胶原蛋白含量和纤维化调节的影响。

结果

SALL4的高表达显著促进了宫颈癌细胞的增殖、迁移和黏附。SALL4基因敲除后，迁移和增殖率明显低于HeLa细胞。免疫荧光和体内实验表明，SALL4基因敲除细胞的肿瘤形成能力显著降低，与HeLa细胞相比，增殖和纤维化水平更低。

结论

SALL4的高表达促进宫颈癌进展，而抑制SALL4表达可有效抑制癌症发展。作为一个关键的调节因子，SALL4有潜力成为宫颈癌的治疗靶点，其在宫颈癌治疗中的应用值得进一步探索。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/7df8934b1677/12885_2025_14469_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/ed7b6ad58f3a/12885_2025_14469_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/2942963f5a5a/12885_2025_14469_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/5032be30f967/12885_2025_14469_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/7df8934b1677/12885_2025_14469_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/ed7b6ad58f3a/12885_2025_14469_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/2942963f5a5a/12885_2025_14469_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/5032be30f967/12885_2025_14469_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/52ae/12210988/7df8934b1677/12885_2025_14469_Fig4_HTML.jpg

相似文献

1
Single-cell sequencing reveals the role of SALL4 in cervical cancer development.单细胞测序揭示了SALL4在宫颈癌发展中的作用。
BMC Cancer. 2025 Jul 1;25(1):1086. doi: 10.1186/s12885-025-14469-2.
2
Dual functions of the ΔNp63-miR-141-3p-YAP1 regulatory axis in cervical cancer progression are dependent on histological subtype.ΔNp63-miR-141-3p-YAP1调控轴在宫颈癌进展中的双重功能取决于组织学亚型。
Sci Rep. 2025 Jul 2;15(1):23155. doi: 10.1038/s41598-025-07237-6.
3
circ-NOLC1 inhibits the development of cervical cancer by regulating miR-330-5p-PALM signaling axis.环状非编码RNA NOLC1通过调控miR-330-5p-PALM信号轴抑制宫颈癌的发展。
Hereditas. 2025 Jun 18;162(1):108. doi: 10.1186/s41065-025-00478-5.
4
Investigation of the mechanism of euphornin against cervical cancer using network pharmacology.基于网络药理学对优福宁抗宫颈癌作用机制的研究
Sci Rep. 2025 Jul 2;15(1):23497. doi: 10.1038/s41598-025-04522-2.
5
Correlation of senescence-related gene FEN1 on neuroblastoma progression and cisplatin chemotherapy sensitivity.衰老相关基因FEN1与神经母细胞瘤进展及顺铂化疗敏感性的相关性
Oncol Res. 2025 Jun 26;33(7):1695-1708. doi: 10.32604/or.2025.060021. eCollection 2025.
6
MIR155HG promotes metastasis and cisplatin resistance of cervical cancer cells by regulating the miR-409-3p and ZEB1 axis.MIR155HG通过调控miR-409-3p和ZEB1轴促进宫颈癌细胞的转移和顺铂耐药。
Sci Rep. 2025 Jul 1;15(1):20541. doi: 10.1038/s41598-025-08727-3.
7
EIF3B‑METTL3 complex promotes cell proliferation, invasion and EGFR/AKT signaling in cervical cancer.EIF3B-METTL3复合物促进宫颈癌的细胞增殖、侵袭及EGFR/AKT信号传导。
Oncol Rep. 2025 Sep;54(3). doi: 10.3892/or.2025.8936. Epub 2025 Jun 27.
8
Caveolin-1 inhibits the proliferation and invasion of lung adenocarcinoma via EGFR degradation.小窝蛋白-1通过表皮生长因子受体（EGFR）降解抑制肺腺癌的增殖和侵袭。
Sci Rep. 2025 Jul 1;15(1):21654. doi: 10.1038/s41598-025-05259-8.
9
[CRISPR-Cas9-mediated CDC20 gene knockout inhibits cervical cancer cell proliferation, invasion and metastasis].[CRISPR-Cas9介导的CDC20基因敲除抑制宫颈癌细胞增殖、侵袭和转移]
Nan Fang Yi Ke Da Xue Xue Bao. 2025 Jun 20;45(6):1200-1211. doi: 10.12122/j.issn.1673-4254.2025.06.09.
10
Gap junction protein beta 5 interacts with Gαi3 to promote Akt activation and cervical cancer cell growth.缝隙连接蛋白β5与Gαi3相互作用以促进Akt激活和宫颈癌细胞生长。
Cell Death Dis. 2025 Jun 19;16(1):461. doi: 10.1038/s41419-025-07768-w.

引用本文的文献

1
Identification of malignant cells in single-cell transcriptomics data.单细胞转录组学数据中恶性细胞的识别。
Commun Biol. 2025 Aug 22;8(1):1264. doi: 10.1038/s42003-025-08695-4.

本文引用的文献

1
Endothelial cells in tumor microenvironment: insights and perspectives.肿瘤微环境中的内皮细胞：深入了解和展望。
Front Immunol. 2024 Feb 15;15:1367875. doi: 10.3389/fimmu.2024.1367875. eCollection 2024.
2
SALL4 promotes cancer stem-like cell phenotype and radioresistance in oral squamous cell carcinomas via methyltransferase-like 3-mediated m6A modification.SALL4 通过甲基转移酶样蛋白 3 介导的 m6A 修饰促进口腔鳞状细胞癌中的肿瘤干细胞样表型和放射抵抗。
Cell Death Dis. 2024 Feb 14;15(2):139. doi: 10.1038/s41419-024-06533-9.
3
Single-cell RNA sequencing of cervical exfoliated cells reveals potential biomarkers and cellular pathogenesis in cervical carcinogenesis.
单细胞 RNA 测序分析宫颈脱落细胞揭示宫颈癌发生中的潜在生物标志物和细胞发病机制。
Cell Death Dis. 2024 Feb 12;15(2):130. doi: 10.1038/s41419-024-06522-y.
4
Pan-cancer spatially resolved single-cell analysis reveals the crosstalk between cancer-associated fibroblasts and tumor microenvironment.泛癌空间分辨单细胞分析揭示了肿瘤相关成纤维细胞与肿瘤微环境之间的相互作用。
Mol Cancer. 2023 Oct 13;22(1):170. doi: 10.1186/s12943-023-01876-x.
5
Comprehensive analysis of scRNA-Seq and bulk RNA-Seq reveals dynamic changes in the tumor immune microenvironment of bladder cancer and establishes a prognostic model.综合分析 scRNA-Seq 和 bulk RNA-Seq 揭示膀胱癌肿瘤免疫微环境的动态变化，并建立一个预后模型。
J Transl Med. 2023 Mar 27;21(1):223. doi: 10.1186/s12967-023-04056-z.
6
Spatiotemporally deciphering the mysterious mechanism of persistent HPV-induced malignant transition and immune remodelling from HPV-infected normal cervix, precancer to cervical cancer: Integrating single-cell RNA-sequencing and spatial transcriptome.从 HPV 感染的正常宫颈、癌前病变到宫颈癌，解析 HPV 持续诱导恶性转化和免疫重塑的时空奥秘：单细胞 RNA 测序和空间转录组学的整合。
Clin Transl Med. 2023 Mar;13(3):e1219. doi: 10.1002/ctm2.1219.
7
SALL4 activates PI3K/AKT signaling pathway through targeting PTEN, thus facilitating migration, invasion and proliferation of hepatocellular carcinoma cells.SALL4 通过靶向 PTEN 激活 PI3K/AKT 信号通路，从而促进肝癌细胞的迁移、侵袭和增殖。
Aging (Albany NY). 2022 Dec 26;14(24):10081-10092. doi: 10.18632/aging.204446.
8
Liver tumour immune microenvironment subtypes and neutrophil heterogeneity.肝肿瘤免疫微环境亚型与中性粒细胞异质性
Nature. 2022 Dec;612(7938):141-147. doi: 10.1038/s41586-022-05400-x. Epub 2022 Nov 9.
9
Characterization of cancer-related fibroblasts (CAF) in hepatocellular carcinoma and construction of CAF-based risk signature based on single-cell RNA-seq and bulk RNA-seq data.肝细胞癌中癌相关成纤维细胞（CAF）的特征分析及基于单细胞 RNA-seq 和 bulk RNA-seq 数据构建 CAF 风险特征。
Front Immunol. 2022 Sep 23;13:1009789. doi: 10.3389/fimmu.2022.1009789. eCollection 2022.
10
Sall4 Guides p53-Mediated Enhancer Interference upon DNA Damage in Mouse Embryonic Stem Cells.Sall4 在 DNA 损伤时介导 p53 介导的增强子干扰作用于小鼠胚胎干细胞。
Stem Cells. 2022 Nov 29;40(11):1008-1019. doi: 10.1093/stmcls/sxac058.