Liaw Y F, Chu C M, Lin L H, Ling C M, Huang M J
Scand J Infect Dis. 1985;17(3):251-7. doi: 10.3109/inf.1985.17.issue-3.03.
The standard radioimmunoassay for anti-HBc (CORAB) was modified for the differential detection of anti-HBc IgM by incorporation of a step in which anti-HBc IgG was preferentially absorbed by Staphylococcus aureus cells (Protein A). The ratio (R) of anti-HBc IgM to total anti-HBc was evaluated by computing the ratio of sample cpm's after and before protein A absorption. The R values of acute B hepatitis ranged from 0.9 to 2.1 (mean 1.3 +/- 0.3) while those of chronic HBsAg carriers ranged from 3.1 to 8.3 (mean 4.9 +/- 1.1). Adopting 2.1 as the upper limit of R value for acute B infection, this modified CORAB was shown to have excellent correlation with enzyme immunoassay, and to be capable of differentiating acute from persistent HBV infection in HBsAg positive patients, and discriminating acute B hepatitis from non-A, non-B hepatitis in HBsAg negative but anti-HBc positive acute hepatitis.
用于抗-HBc检测的标准放射免疫分析法（CORAB）经过了改良，通过加入一个步骤来实现抗-HBc IgM的鉴别检测，该步骤是利用金黄色葡萄球菌细胞（蛋白A）优先吸附抗-HBc IgG。抗-HBc IgM与总抗-HBc的比值（R）通过计算蛋白A吸附前后样品每分钟计数（cpm）的比值来评估。急性乙型肝炎的R值范围为0.9至2.1（平均1.3±0.3），而慢性HBsAg携带者的R值范围为3.1至8.3（平均4.9±1.1）。采用2.1作为急性乙型感染R值的上限，这种改良的CORAB被证明与酶免疫分析法具有良好的相关性，并且能够区分HBsAg阳性患者的急性HBV感染与持续性HBV感染，以及在HBsAg阴性但抗-HBc阳性的急性肝炎患者中鉴别急性乙型肝炎与非甲非乙型肝炎。
